Linked Life Data

13129357

Source:http://linkedlifedata.com/resource/pubmed/id/13129357

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
38
pubmed:dateCreated
2003-9-17
pubmed:abstractText
We report herein a versatile postsynthetic modification of on-column oligodeoxynucleotides (ODNs) using a copper-catalyzed oxidative acetylenic coupling reaction. Hexamers supported on resins via a methylamino-modified linker were prepared, and on-column modifications of ODNs were examined. ArgoPore resin proved to be the best choice for the modification, and introduction of functional molecules, such as anthraquinone, biotin, and fluorescein, resulted in good yields at not only the 5'-terminal but also the internal 3'-end of the ODNs. This method is applicable to the modification of 12mer ODN consisting of a random sequence. The resulting ODN9 possessing fluorescein at its 5'-terminal acts as a non-RI primer for primer extension assays using the Klenow fragment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0002-7863
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11545-52
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
A versatile modification of on-column oligodeoxynucleotides using a copper-catalyzed oxidative acetylenic coupling reaction.
pubmed:affiliation
Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan. noriaki@pharm.hokudai.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't