Linked Life Data

1312270

Source:http://linkedlifedata.com/resource/pubmed/id/1312270

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-4-15
pubmed:abstractText
Epstein-Barr virus nuclear antigen 1 (EBNA 1) has been shown to be a sequence-specific DNA binding protein that is required for the replication of episomal elements carrying the viral origin of DNA replication, oriP, as well as for the activation of a specific transcriptional enhancer. We have constructed and analyzed a series of deletion and nonsense mutants in a cloned copy of the EBNA 1 gene and have tested mutant peptides for the ability (a) to bind to a synthetic oligonucleotide containing a consensus EBNA 1 binding site, (b) to activate the EBNA 1-specific enhancer, and (c) to drive replication of an oriP-bearing plasmid in a transient replication assay. The presence of a DNA binding domain in the carboxy-terminal third of the protein was confirmed. Interestingly, neither the acidic tail nor the Gly-Ala copolymer of EBNA 1 contributes significantly to binding. In addition to sequences in the carboxy-terminal portion of the protein, our data indicate that sequences in the amino-terminal portion of the polypeptide affect the binding of EBNA 1 to its target sequence. Further, we show that EBNA 1 binds to its recognition sequence as a dimer. Results of transient expression assays indicate that the ability of EBNA 1 species to activate the transcriptional enhancer and to drive the replication of oriP plasmids is directly dependent on the ability of the polypeptides to bind to the EBNA 1 consensus binding sequence.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:volume
187
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
591-603
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:1312270-Antigens, Viral, pubmed-meshheading:1312270-Base Sequence, pubmed-meshheading:1312270-Cloning, Molecular, pubmed-meshheading:1312270-DNA Mutational Analysis, pubmed-meshheading:1312270-DNA Replication, pubmed-meshheading:1312270-DNA-Binding Proteins, pubmed-meshheading:1312270-Enhancer Elements, Genetic, pubmed-meshheading:1312270-Epstein-Barr Virus Nuclear Antigens, pubmed-meshheading:1312270-Gene Expression Regulation, Viral, pubmed-meshheading:1312270-Herpesvirus 4, Human, pubmed-meshheading:1312270-Oligodeoxyribonucleotides, pubmed-meshheading:1312270-Plasmids, pubmed-meshheading:1312270-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:1312270-Structure-Activity Relationship, pubmed-meshheading:1312270-Transcription, Genetic, pubmed-meshheading:1312270-Transcriptional Activation
pubmed:year
1992
pubmed:articleTitle
DNA binding activity is required for EBNA 1-dependent transcriptional activation and DNA replication.
pubmed:affiliation
Department of Biology, Northeastern University, Boston, Massachusetts 02115.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't