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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1992-3-23
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pubmed:databankReference |
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pubmed:abstractText |
A full-length cDNA clone encoding a glutamate receptor was isolated from a human brain cDNA library, and the gene product was characterized after expression in Xenopus oocytes. Degenerate PCR primers to conserved regions of published rat brain glutamate receptor sequences amplified a 1-kilobase fragment from a human brain cDNA library. This fragment was used as a probe for subsequent hybridization screening. Two clones were isolated that, based on sequence information, code for different receptors: a 3-kilobase clone, HBGR1, contains a full-length glutamate receptor cDNA highly homologous to the rat brain clone GluR1, and a second clone, HBGR2, contains approximately two-thirds of the coding region of a receptor homologous to rat brain clone GluR2. Southern and PCR analysis of a somatic cell-hybrid panel mapped HBGR1 to human chromosome 5q31.3-33.3 and mapped HBGR2 to chromosome 4q25-34.3. Xenopus oocytes injected with in vitro-synthesized HBGR1 cRNA expressed currents activated by glutamate receptor agonists with the following specificity sequence: domoate greater than kainate much greater than quisqualate greater than or equal to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid greater than or equal to L-glutamate much greater than N-methyl-D-aspartate. The kainate-elicited currents were specifically blocked by 6-cyano-7-nitroquinoxaline-2,3-dione but were insensitive to 2-amino-5-phosphonovalerate and kynurenic acid. These results indicate that clone HBGR1 codes for a glutamate receptor of the kainate subtype cognate to members of the glutamate receptor family from rodent brain.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-1648177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-1652753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-1673850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-1697342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-1699275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-1699567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-1710829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-1827708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-1834949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-1847995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-1925546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-1970230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-2166337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-2168579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-2188577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-2432468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-2480522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-2494655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-2543272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-2558391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-2856092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-2884625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-3186754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-3472723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-3526287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-3860826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-6207484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-6323990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-6546423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311100-9732752
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
89
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pubmed:geneSymbol |
HBGR1,
HBGR2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1443-7
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1311100-Amino Acid Sequence,
pubmed-meshheading:1311100-Animals,
pubmed-meshheading:1311100-Chromosome Mapping,
pubmed-meshheading:1311100-Chromosomes, Human, Pair 4,
pubmed-meshheading:1311100-Chromosomes, Human, Pair 5,
pubmed-meshheading:1311100-Cloning, Molecular,
pubmed-meshheading:1311100-DNA,
pubmed-meshheading:1311100-Gene Expression,
pubmed-meshheading:1311100-Humans,
pubmed-meshheading:1311100-Kainic Acid,
pubmed-meshheading:1311100-Molecular Sequence Data,
pubmed-meshheading:1311100-Polymerase Chain Reaction,
pubmed-meshheading:1311100-Receptors, Glutamate,
pubmed-meshheading:1311100-Receptors, Neurotransmitter,
pubmed-meshheading:1311100-Xenopus laevis
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pubmed:year |
1992
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pubmed:articleTitle |
Molecular cloning, chromosomal mapping, and functional expression of human brain glutamate receptors.
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pubmed:affiliation |
Department of Biology, University of California, San Diego, La Jolla 92093.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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