Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1992-3-23
pubmed:abstractText
Retinoic acid receptors are ligand-dependent transcription factors that stimulate gene transcription from promoters containing retinoic acid or thyroid hormone response elements. We describe a high-affinity binding site from the rat oxytocin promoter that mediates negative transcriptional regulation by the retinoic acid receptor. To examine whether strong, constitutive transactivation domains would be capable of stimulating gene transcription when bound to this DNA binding site that normally mediates transcriptional repression, we fused the transactivation domain of the herpes simplex viral protein VP16 to the amino terminus of the retinoic acid receptor and tested the activity of the chimeric protein on the negative retinoic acid response element. This chimeric retinoic acid receptor acted as a strong, constitutive transactivator when bound to promoters containing palindromic thyroid hormone/retinoic acid response elements but surprisingly it still repressed gene transcription when bound to promoters containing the oxytocin-negative retinoic acid response element. These results suggest that a negative DNA binding site itself can inhibit the function of even potent constitutive transactivation domains, and provide evidence that tethering of a constitutive transactivation domain to DNA is insufficient to activate gene transcription.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-1648728, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-1846049, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2108152, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2119054, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2153268, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2164891, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2169351, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2169352, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2169353, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2499084, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2555064, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2556699, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2825025, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2825036, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2836738, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2838908, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2848197, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2905023, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-2994050, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-3024155, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-3043662, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-3047590, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-3050531, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-3396073, http://linkedlifedata.com/resource/pubmed/commentcorrection/1311087-3907859
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1209-13
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed-meshheading:1311087-Animals, pubmed-meshheading:1311087-Base Sequence, pubmed-meshheading:1311087-Carrier Proteins, pubmed-meshheading:1311087-Cells, Cultured, pubmed-meshheading:1311087-Cercopithecus aethiops, pubmed-meshheading:1311087-DNA-Binding Proteins, pubmed-meshheading:1311087-Gene Expression Regulation, pubmed-meshheading:1311087-Molecular Sequence Data, pubmed-meshheading:1311087-Nuclear Proteins, pubmed-meshheading:1311087-Oligodeoxyribonucleotides, pubmed-meshheading:1311087-Oxytocin, pubmed-meshheading:1311087-Promoter Regions, Genetic, pubmed-meshheading:1311087-Rats, pubmed-meshheading:1311087-Receptors, Retinoic Acid, pubmed-meshheading:1311087-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:1311087-Transcription, Genetic, pubmed-meshheading:1311087-Transcriptional Activation, pubmed-meshheading:1311087-Tretinoin
pubmed:year
1992
pubmed:articleTitle
A negative retinoic acid response element in the rat oxytocin promoter restricts transcriptional stimulation by heterologous transactivation domains.
pubmed:affiliation
Eukaryotic Regulatory Biology Program, School of Medicine, University of California, San Diego, La Jolla 92093-0648.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't