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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0010453,
umls-concept:C0015127,
umls-concept:C0022677,
umls-concept:C0033634,
umls-concept:C0052088,
umls-concept:C0205191,
umls-concept:C0443252,
umls-concept:C1314792,
umls-concept:C1879547,
umls-concept:C1948023
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pubmed:issue |
2
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pubmed:dateCreated |
1992-3-18
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pubmed:abstractText |
To examine the role of protein kinase C as a chronic regulator of proximal tubule Na/H antiporter activity, the effect of phorbol 12-myristate 13-acetate (PMA) on the Na/H antiporter was studied in cultured proximal tubule cells. Short-term activation of protein kinase C by 5 min exposure to PMA caused an acute increase in Na/H antiporter activity that was not prevented by cycloheximide or actinomycin D and did not persist 24 h later. Long-term activation of protein kinase C by 2 h exposure to PMA caused a dose-dependent increase in Na/H antiporter activity 24 h later. This latter effect was due to protein kinase C activation in that it was inhibited by sphingosine and was not seen with 4 alpha-PMA, an inactive analogue. The chronic effect of PMA was inhibited by 10 nM actinomycin D or 7 microM cycloheximide. Proximal tubule cells exposed to PMA for 2 h demonstrated a two- to threefold increase in Na/H antiporter mRNA (mRNANa/H) abundance 4 h later. In conclusion, short-term activation of protein kinase C leads to a transient increase in Na/H antiporter activity that is independent of transcription and translation, whereas long-term activation of protein kinase C causes a persistent increase in antiporter activity that is dependent on transcription and translation and is associated with increased mRNANa/H abundance. This latter effect may mediate increased Na/H antiporter activity in a number of chronic conditions.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-1658050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-1849958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2040601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2120446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2154036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2158991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2162055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2170445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2330986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2404291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2422432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2454509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2469963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2536298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2537851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2554050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2564256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2821407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2827521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2844858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2847049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2874497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2901105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2917998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2996368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-2999293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-3009822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-3020025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-3020428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-3027347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-3032984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-3038953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-3115982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-3141060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-3217240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-3775383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-3885217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-3944131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-3953824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-6087681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-6119031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-6282141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-6309012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-6320201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-6439738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-6511911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1310692-7012859
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
365-72
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1310692-Animals,
pubmed-meshheading:1310692-Carrier Proteins,
pubmed-meshheading:1310692-Cells, Cultured,
pubmed-meshheading:1310692-Enzyme Activation,
pubmed-meshheading:1310692-Kidney Tubules, Proximal,
pubmed-meshheading:1310692-Male,
pubmed-meshheading:1310692-Protein Biosynthesis,
pubmed-meshheading:1310692-Protein Kinase C,
pubmed-meshheading:1310692-RNA, Messenger,
pubmed-meshheading:1310692-Rabbits,
pubmed-meshheading:1310692-Sodium-Hydrogen Antiporter,
pubmed-meshheading:1310692-Tetradecanoylphorbol Acetate,
pubmed-meshheading:1310692-Transcription, Genetic
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pubmed:year |
1992
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pubmed:articleTitle |
Long-term activation of protein kinase c causes chronic Na/H antiporter stimulation in cultured proximal tubule cells.
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