Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-3-3
pubmed:abstractText
The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) at 10(-9) M stimulated the formation of osteoclast-like multinucleated cells (MNCs) in the presence of 1 alpha,25-dihydroxyvitamin D3 in rat bone marrow cultures. However, at 10(-7) M, it clearly inhibited 1 alpha,25-dihydroxyvitamin D3-dependent osteoclast-like MNC formation at 6 days of culture. In cultures treated with 10(-7) M TPA, numerous MNCs that lack the marker enzyme tartrate-resistant acid phosphatase (TRAP) were formed. These TRAP-negative MNCs had neither receptors for calcitonin nor dentine-resorbing activity. The reactivity of the cells against antirat macrophage antibodies was completely different from that of authentic osteoclasts. These data suggest that TRAP-negative MNCs formed in the presence of 10(-7) M TPA are macrophage polykaryons. Time-course studies showed that 10(-7) M TPA stimulated osteoclast-like MNC formation at 4 days of culture, but these osteoclast-like MNCs were converted to TRAP-negative MNCs. Furthermore, 1-(5-isoquinolinyl-sulfonyl)2-methylpiperazine (H-7), a protein kinase-C inhibitor, inhibited osteoclast-like MNC formation in a dose-dependent fashion. These results suggest that activation of protein kinase-C may play a role in osteoclast differentiation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/1-(5-Isoquinolinesulfonyl)-2-Methylp..., http://linkedlifedata.com/resource/pubmed/chemical/Acid Phosphatase, http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers, http://linkedlifedata.com/resource/pubmed/chemical/Calcitonin, http://linkedlifedata.com/resource/pubmed/chemical/Calcitriol, http://linkedlifedata.com/resource/pubmed/chemical/Carcinogens, http://linkedlifedata.com/resource/pubmed/chemical/Isoquinolines, http://linkedlifedata.com/resource/pubmed/chemical/Phorbol Esters, http://linkedlifedata.com/resource/pubmed/chemical/Piperazines, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Calcitonin, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface, http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate, http://linkedlifedata.com/resource/pubmed/chemical/phorbol-12,13-didecanoate
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
130
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
577-84
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:1310276-1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine, pubmed-meshheading:1310276-Acid Phosphatase, pubmed-meshheading:1310276-Animals, pubmed-meshheading:1310276-Biological Markers, pubmed-meshheading:1310276-Bone Marrow, pubmed-meshheading:1310276-Bone Marrow Cells, pubmed-meshheading:1310276-Bone Resorption, pubmed-meshheading:1310276-Calcitonin, pubmed-meshheading:1310276-Calcitriol, pubmed-meshheading:1310276-Carcinogens, pubmed-meshheading:1310276-Cell Differentiation, pubmed-meshheading:1310276-Cells, Cultured, pubmed-meshheading:1310276-Isoquinolines, pubmed-meshheading:1310276-Kinetics, pubmed-meshheading:1310276-Macrophages, pubmed-meshheading:1310276-Microscopy, Electron, Scanning, pubmed-meshheading:1310276-Models, Biological, pubmed-meshheading:1310276-Osteoclasts, pubmed-meshheading:1310276-Phorbol Esters, pubmed-meshheading:1310276-Piperazines, pubmed-meshheading:1310276-Protein Kinase C, pubmed-meshheading:1310276-Rats, pubmed-meshheading:1310276-Rats, Inbred Strains, pubmed-meshheading:1310276-Receptors, Calcitonin, pubmed-meshheading:1310276-Receptors, Cell Surface, pubmed-meshheading:1310276-Tetradecanoylphorbol Acetate
pubmed:year
1992
pubmed:articleTitle
12-O-tetradecanoylphorbol-13-acetate inhibits osteoclast-like cell differentiation in rat bone marrow cultures by inducing macrophage polykaryons.
pubmed:affiliation
Second Department of Anatomy, Faculty of Dentistry, Kyushu University, Fukuoka, Japan.
pubmed:publicationType
Journal Article, Comparative Study