Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4-5
pubmed:dateCreated
1993-10-21
pubmed:abstractText
The technique for foreign gene transfer in fish is becoming a novel method for genetic engineers to produce useful transgenic fish as well as for experimental purposes. Our studies of transgenic fish have been focused on methods of gene transfer and regulatory elements for transgene expression. I describe the characteristics of 3 gene transfer methods we have established (i.e., microinjection into fertilized eggs, microinjection into oocytes, and electroporation). Also described is a series of experiments to estimate activities of several promoters and enhancers in a fish cell line. Finally, experiments to examine activities of the elements shown to be active in the cell line in fish embryos and fry are described. SV2, miw, and metallothioneine promoters of trout and mouse were shown to be active in these experiments.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1053-6426
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:geneSymbol
lacZ, rtMT-A
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
266-70
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:articleTitle
Expression of reporter genes introduced by microinjection and electroporation in fish embryos and fry.
pubmed:affiliation
Central Research Laboratory, Nippon Suisan Kaisha, Ltd., Tokyo, Japan.
pubmed:publicationType
Journal Article, Review