Linked Life Data

1303744

Source:http://linkedlifedata.com/resource/pubmed/id/1303744

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1993-7-1
pubmed:abstractText
Macromolecular crystal growth experiments, using satellite tobacco mosaic virus (STMV) and canavalin from jack beans as samples, were conducted on a US Space Shuttle mission designated International Microgravity Laboratory--1 (IML-1), flown January 22-29, 1992. Parallel experiments using identical samples were carried out in both a vapor diffusion-based device (PCG) and a liquid-liquid diffusion-based instrument (CRYOSTAT). The experiments in each device were run at 20-22 degrees C and at colder temperatures. Crystals were grown in virtually every trial, but the characteristics of the crystals were highly dependent on the crystallization technique employed and the temperature experience of the sample. In general, very good results, based on visual inspection of the crystals, were obtained in both PCG and CRYOSTAT. Unusually impressive results were, however, achieved for STMV in the CRYOSTAT instrument. STMV crystals grown in microgravity by liquid-liquid diffusion were more than 10-fold greater in total volume than any STMV crystals previously grown in the laboratory. X-ray diffraction data collected from eight STMV crystals grown in CRYOSTAT demonstrated a substantial improvement in diffraction quality over the entire resolution range when compared to data from crystals grown on Earth. In addition, the extent of the diffraction pattern for the STMV crystals grown in space extended to 1.8 A resolution, whereas the best crystals that were ever grown under conditions of Earth's gravity produced data limited to 2.3 A resolution. Other observations indicate that the growth of macromolecular crystals is indeed influenced by the presence or absence of gravity. These observations further suggest, consistent with earlier results, that the elimination of gravity provides a more favorable environment for such processes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1303744-1180565, http://linkedlifedata.com/resource/pubmed/commentcorrection/1303744-16662638, http://linkedlifedata.com/resource/pubmed/commentcorrection/1303744-1731967, http://linkedlifedata.com/resource/pubmed/commentcorrection/1303744-17836869, http://linkedlifedata.com/resource/pubmed/commentcorrection/1303744-17837944, http://linkedlifedata.com/resource/pubmed/commentcorrection/1303744-2185018, http://linkedlifedata.com/resource/pubmed/commentcorrection/1303744-2488475, http://linkedlifedata.com/resource/pubmed/commentcorrection/1303744-2510297, http://linkedlifedata.com/resource/pubmed/commentcorrection/1303744-2585487, http://linkedlifedata.com/resource/pubmed/commentcorrection/1303744-2718378, http://linkedlifedata.com/resource/pubmed/commentcorrection/1303744-4369031, http://linkedlifedata.com/resource/pubmed/commentcorrection/1303744-4625692, http://linkedlifedata.com/resource/pubmed/commentcorrection/1303744-4733844, http://linkedlifedata.com/resource/pubmed/commentcorrection/1303744-5497144
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0961-8368
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1254-68
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Macromolecular crystal growth experiments on International Microgravity Laboratory--1.
pubmed:affiliation
Department of Biochemistry, University of California, Riverside 92521.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.