Linked Life Data

1303096

Source:http://linkedlifedata.com/resource/pubmed/id/1303096

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-6-23
pubmed:abstractText
Antigens injected into the anterior chamber of the eye generate a deviant immune response (Anterior Chamber Associated Immune Deviation--ACAID) characterized by impaired delayed hypersensitivity and lack of complement-fixing antibodies. Both the antigen-containing eye and an intact spleen are required for ACAID induction. The eye's contribution takes the form of an antigen-specific, ACAID-inducing signal associated with blood-borne F4/80+ macrophages. Macrophages capable of carrying an ACAID-inducing signal can be harvested directly from the iris and ciliary body, or can be created by exposing F4/80+ peritoneal exudate cells to aqueous humor or to supernatants of cultured iris and ciliary body cells. In the present study, F4/80-bearing macrophages from the peritoneal cavity were exposed in vitro to supernatants of cultured iris and ciliary body cells and then labelled with PKH-2 prior to intravenous infusion into naive, syngeneic recipients. The pattern of distribution of these cells in spleen, lymph nodes, and blood was compared with similarly labeled cells exposed in vitro to medium alone. The results reveal that, compared to conventional peritoneal exudate cells, macrophages that are endowed with ACAID-inducing properties (by exposure to supernatants of iris and ciliary body cell cultures) preferentially migrate to the spleen following their intravenous inoculation into syngeneic recipients. Moreover, when injected intravenously into asplenic mice, macrophages with ACAID-inducing potential accumulate excessively (compared to their conventional counterparts) in the peripheral blood.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0896-0623
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
130-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:articleTitle
Macrophages capable of inducing anterior chamber associated immune deviation demonstrate spleen-seeking migratory properties.
pubmed:affiliation
Department of Microbiology and Immunology, University of Miami School of Medicine, FL 33101.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.