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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0017337,
umls-concept:C0017431,
umls-concept:C0020792,
umls-concept:C0023745,
umls-concept:C0024779,
umls-concept:C0242772,
umls-concept:C0332325,
umls-concept:C0441513,
umls-concept:C0683598,
umls-concept:C1521991,
umls-concept:C1527177,
umls-concept:C1527180,
umls-concept:C1704319,
umls-concept:C2745888
|
| pubmed:issue |
6
|
| pubmed:dateCreated |
1993-6-11
|
| pubmed:abstractText |
A dominant allele at the Mi locus on chromosome 6 of tomato (Lycopersicon esculentum Mill) confers resistance to three species of root-knot nematodes (Meloidogyne). The resistance, which is associated with a localized necrotic response, was originally introduced into tomato from the wild species Lycopersicon peruvianum. As a step towards the molecular cloning of Mi, we have identified closely linked DNA markers from both cDNA and genomic DNA libraries as restriction fragment length polymorphisms (RFLPs). DNA from tomato populations segregating for nematode resistance was analyzed to generate a high-resolution genetic map of this region. Additional information on gene order was obtained by comparing the size of the introgressed L. peruvianum chromosomal segment within a collection of nematode-resistant tomato lines. Among the four cDNA markers that are tightly linked to Mi, three are dominant, i.e. L. peruvianum-specific. One cDNA marker corresponds to a gene family comprising 20-30 members, one of which is diagnostic for all nematode-resistant genotypes tested. The presence of non-homologous sequences around the Mi gene may contribute to the suppression of recombination in this region of the genome in crosses heterozygous for Mi. The potential of 'walking' from closely linked markers to Mi is discussed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0960-7412
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
2
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
971-82
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:1302643-Animals,
pubmed-meshheading:1302643-Base Sequence,
pubmed-meshheading:1302643-Chromosome Mapping,
pubmed-meshheading:1302643-Crosses, Genetic,
pubmed-meshheading:1302643-Fruit,
pubmed-meshheading:1302643-Genes, Dominant,
pubmed-meshheading:1302643-Genes, Plant,
pubmed-meshheading:1302643-Genetic Linkage,
pubmed-meshheading:1302643-Genetic Markers,
pubmed-meshheading:1302643-Genetic Variation,
pubmed-meshheading:1302643-Genotype,
pubmed-meshheading:1302643-Immunity, Innate,
pubmed-meshheading:1302643-Molecular Sequence Data,
pubmed-meshheading:1302643-Nematode Infections,
pubmed-meshheading:1302643-Recombination, Genetic,
pubmed-meshheading:1302643-Species Specificity,
pubmed-meshheading:1302643-Tylenchoidea,
pubmed-meshheading:1302643-Virulence
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
The root-knot nematode resistance gene (Mi) in tomato: construction of a molecular linkage map and identification of dominant cDNA markers in resistant genotypes.
|
| pubmed:affiliation |
Department of Nematology, University of California, Davis 95616.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|