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pubmed-article:1302504pubmed:abstractTextTwo enzyme forms of endonuclease (Sm 1 and Sm 2) strain B10M1 in 60 and 100 mg respectively have been isolated from the culture fluid Serratia marcescens. The chromatographic and electrophoretic properties and N-terminal amino acid residues are different for both enzymes. The purification procedure consists of dialysis and ion-exchange chromatography on DEAE- and phosphocellulose. The yield of nucleases Sm1 and Sm2 are 14% and 28% respectively. The antigenic differences of nucleases Sm1 and Sm2 have been found by cross immunoenzyme analysis.lld:pubmed
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pubmed-article:1302504pubmed:articleTitle[The isolation of nucleases Sm1 and Sm2 in preparative amounts and a study of their antigenicity].lld:pubmed
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pubmed-article:1302504pubmed:publicationTypeEnglish Abstractlld:pubmed