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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1993-6-17
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pubmed:abstractText |
Two enzyme forms of endonuclease (Sm 1 and Sm 2) strain B10M1 in 60 and 100 mg respectively have been isolated from the culture fluid Serratia marcescens. The chromatographic and electrophoretic properties and N-terminal amino acid residues are different for both enzymes. The purification procedure consists of dialysis and ion-exchange chromatography on DEAE- and phosphocellulose. The yield of nucleases Sm1 and Sm2 are 14% and 28% respectively. The antigenic differences of nucleases Sm1 and Sm2 have been found by cross immunoenzyme analysis.
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pubmed:language |
rus
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0470-4606
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
65-70
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pubmed:dateRevised |
2009-11-11
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pubmed:meshHeading |
pubmed-meshheading:1302504-Animals,
pubmed-meshheading:1302504-Antigens, Bacterial,
pubmed-meshheading:1302504-Chromatography, DEAE-Cellulose,
pubmed-meshheading:1302504-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1302504-Endodeoxyribonucleases,
pubmed-meshheading:1302504-Endoribonucleases,
pubmed-meshheading:1302504-Immunization,
pubmed-meshheading:1302504-Immunization, Secondary,
pubmed-meshheading:1302504-Immunoelectrophoresis, Two-Dimensional,
pubmed-meshheading:1302504-Rabbits,
pubmed-meshheading:1302504-Serratia marcescens
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pubmed:year |
1992
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pubmed:articleTitle |
[The isolation of nucleases Sm1 and Sm2 in preparative amounts and a study of their antigenicity].
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pubmed:publicationType |
Journal Article,
English Abstract
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