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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1993-5-28
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pubmed:abstractText |
The effect of progesterone was studied on the sulfate entry in glandular epithelial cells of guinea-pig endometrium subcultured in bicameral chambers on matrix-coated filters in a chemically defined medium. At post-confluency (8 days of subculture), cells were treated with 10 nM estradiol alone or in association with various concentrations of progesterone. Optimal progesterone action was at a 16 h incubation time and a 10 nM hormonal concentration. Progesterone increased in a dose-dependent fashion the sulfate uptake specifically in glandular epithelial cells, preferentially from the basal surface. Progesterone effect on the sulfate uptake occurred only in estradiol-primed epithelial cells and was inhibited by the antiprogestin steroid RU-486. The progesterone-dependent increase in sulfate uptake was inhibited by the inhibitor of anion exchange, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS). At physiological sulfate concentrations, progesterone essentially induces a high-affinity DIDS-sensitive transport system.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4,4'-Diisothiocyanostilbene-2,2'-Dis...,
http://linkedlifedata.com/resource/pubmed/chemical/4-Acetamido-4'-isothiocyanatostilben...,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/Estradiol,
http://linkedlifedata.com/resource/pubmed/chemical/Mifepristone,
http://linkedlifedata.com/resource/pubmed/chemical/Plastics,
http://linkedlifedata.com/resource/pubmed/chemical/Progesterone,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfates
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0303-7207
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
95-102
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1301401-4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid,
pubmed-meshheading:1301401-4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid,
pubmed-meshheading:1301401-Animals,
pubmed-meshheading:1301401-Cells, Cultured,
pubmed-meshheading:1301401-Culture Media, Serum-Free,
pubmed-meshheading:1301401-Dose-Response Relationship, Drug,
pubmed-meshheading:1301401-Drug Interactions,
pubmed-meshheading:1301401-Endometrium,
pubmed-meshheading:1301401-Epithelial Cells,
pubmed-meshheading:1301401-Epithelium,
pubmed-meshheading:1301401-Estradiol,
pubmed-meshheading:1301401-Extracellular Matrix,
pubmed-meshheading:1301401-Female,
pubmed-meshheading:1301401-Guinea Pigs,
pubmed-meshheading:1301401-Kinetics,
pubmed-meshheading:1301401-Mifepristone,
pubmed-meshheading:1301401-Plastics,
pubmed-meshheading:1301401-Progesterone,
pubmed-meshheading:1301401-Sulfates
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pubmed:year |
1992
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pubmed:articleTitle |
Progesterone stimulates sulfate uptake in subcultured endometrial epithelial cells.
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pubmed:affiliation |
Unité de Recherches de Biochimie Hormonale et des Régulations, INSERM U 198, Besançon, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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