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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2003-9-16
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pubmed:abstractText |
Antigenic stimulation of T cells gives rise to short-lived effector cells and long-lived memory cells. We used two stable isotope-labeling techniques to identify kinetically distinct subpopulations of T cells and to determine the effect of advanced infection with HIV-1. Long-term deuterated water (2H2O) incorporation into DNA demonstrated biphasic accrual of total and of memory/effector (m/e)-phenotype but not naive-phenotype T cells, consistent with the presence of short-lived and longer-lived subpopulations within the m/e-phenotype T cell pool. These results were mirrored by biphasic die-away kinetics in m/e- but not naive-phenotype T cells after short-term 2H-glucose labeling. Persistent label retention was observed in a subset of m/e-phenotype T cells (presumably memory T cells), confirming the presence of T cells with very different life spans in humans. In advanced HIV-1 infection, much higher proportions of T cells were short-lived, compared to healthy controls. Effective long-term anti-retroviral therapy restored values to normal. These results provide the first quantitative evidence that long-lived and quiescent T cells do indeed predominate in the T cell pool in humans and determine T cell pool size, as in rodents. The greatest impact of advanced HIV-1 infection is to reduce the generation of long-lived, potential progenitor T cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-10362629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-10376601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-10500289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-10537102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-10537110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-10611356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-10712441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-11017098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-11169402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-11309627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-11452113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-11683577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-11696593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-11700973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-11714623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-11748275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-11807713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-11927927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-12424339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-12434018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-12562673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-12975464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-13875511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-14170408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-2245440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-6605376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-7585008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-8600537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-9348298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-9435257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-9600962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-9870316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12975480-9883844
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
112
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
956-66
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12975480-Anti-HIV Agents,
pubmed-meshheading:12975480-Blood Glucose,
pubmed-meshheading:12975480-CD4-Positive T-Lymphocytes,
pubmed-meshheading:12975480-CD8-Positive T-Lymphocytes,
pubmed-meshheading:12975480-Cell Survival,
pubmed-meshheading:12975480-DNA,
pubmed-meshheading:12975480-Deuterium,
pubmed-meshheading:12975480-HIV Infections,
pubmed-meshheading:12975480-HIV-1,
pubmed-meshheading:12975480-Humans,
pubmed-meshheading:12975480-Immunologic Memory,
pubmed-meshheading:12975480-Lymphocyte Count,
pubmed-meshheading:12975480-Phenotype,
pubmed-meshheading:12975480-T-Lymphocyte Subsets
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pubmed:year |
2003
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pubmed:articleTitle |
Subpopulations of long-lived and short-lived T cells in advanced HIV-1 infection.
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pubmed:affiliation |
University of California, Berkeley, 119 Morgan Hall, Berkeley, California 94720-3104, USA. march@nature.berkeley.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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