Linked Life Data

12974795

Source:http://linkedlifedata.com/resource/pubmed/id/12974795

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2003-9-16
pubmed:abstractText
The functional supertype of HLA-A2 was investigated in the presentation of the A*0201-restricted Flu matrix p58-66 peptide to activate recall CD8+ T-cell response. In healthy Northern Chinese, the HLA-A2 supertype was mainly composed of the six alleles, A*0201 (26.4%), A*0206 (12.7%), A*0203 (8.2%), A*0207 (7.3%), A*0210 (1.8%) and A*0205 (0.9%), as analyzed by PCR using sequence specific primer (PCR-SSP) and sequence based typing (SBT). The IFN-gamma release Elispot assay was employed to assess effector CD8+ T cells. In A*0201-bearing individuals, the CD8+ T-cell response was potent when stimulated with autologous CD8- PBMCs. The frequency of the effector CD8+ T cells was 96.6% with the magnitude of effector CD8+ T cells of 225 SFC/5 x 104 CD8+ T cells and the RI of 25.7. In non-A*0201 individuals, the effector CD8+ T cells were minimally detectable while the peptide was presented by the autologous CD8- PBMCs. However, the induction of the response of CD8+ T cells obtained from non-A*0201 individuals was remarkably improved when the peptide was presented by autologous dendritic cells instead of CD8- PBMCs. The HLA-A2 alleles possessing cross-reactivity in the peptide presentation were mainly of A*0206 and non-A*0201 heterozygotes of A*0206 and A*0210. Moreover, A*0206 as the HLA-A2 functional supertype was further confirmed by tetramer assay. In two A*0206+ donors with CD8+ T-cell response to the peptide, the CD8+ T-cell frequency assessed by specific binding of peptide HLA-A*0201 tetramer was 4.62% and 1.66%, respectively. Thus, our results have substantiated the immunological relevance of the HLA-A2 supertype, which may benefit the design of peptide vaccines with the potential to be applicable in broader populations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0001-2815
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
285-95
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12974795-Alleles, pubmed-meshheading:12974795-Antigen Presentation, pubmed-meshheading:12974795-Antigen-Presenting Cells, pubmed-meshheading:12974795-Antigens, Viral, pubmed-meshheading:12974795-Bone Marrow Cells, pubmed-meshheading:12974795-CD8-Positive T-Lymphocytes, pubmed-meshheading:12974795-China, pubmed-meshheading:12974795-Dendritic Cells, pubmed-meshheading:12974795-HLA-A2 Antigen, pubmed-meshheading:12974795-Histocompatibility Testing, pubmed-meshheading:12974795-Humans, pubmed-meshheading:12974795-Influenza, Human, pubmed-meshheading:12974795-Influenza A virus, pubmed-meshheading:12974795-Interferon-gamma, pubmed-meshheading:12974795-Lymphocyte Activation, pubmed-meshheading:12974795-Peptide Fragments, pubmed-meshheading:12974795-T-Lymphocytes, Cytotoxic, pubmed-meshheading:12974795-Viral Matrix Proteins
pubmed:year
2003
pubmed:articleTitle
Functional supertype of HLA-A2 in the presentation of Flu matrix p58-66 to induce CD8+ T-cell response in a Northern Chinese population.
pubmed:affiliation
Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't