Linked Life Data

12972753

Source:http://linkedlifedata.com/resource/pubmed/id/12972753

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2003-9-15
pubmed:abstractText
The polymerase chain reaction (PCR) is a powerful molecular biologic technique that permits detection and identification of infinitesimal quantities of DNA. It is well suited to the diagnosis of infectious posterior segment ocular inflammatory disease. In this review, the basic biochemistry of PCR, indications for its use, strengths and limitations to the technique, and examples of clinical application of PCR to posterior segment disease will be discussed. Emerging uses of PCR in linking pathogen to disease will also be outlined. After completion of this article, the reader will be able to describe the basic biochemistry and practical application of PCR in the diagnosis of posterior segment inflammatory disease, list the indications, advantages, and disadvantages of the technique, and outline the application of PCR diagnostics to individual differential diagnoses.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0275-004X
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
445-52
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Cme review: polymerase chain reaction diagnostics for posterior segment disease.
pubmed:affiliation
Department of Ophthalmology and Visual Sciences, Department of Molecular Biology and Pharmacology, Washington University Medical School, Campus Box 8096, 660 South Euclid Avenue, St. Louis, MO 63110, USA. vangelder@vision.wustl.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Review, Research Support, Non-U.S. Gov't