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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2003-9-15
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pubmed:abstractText |
A human pituitary cDNA library was screened using a yeast one-hybrid system to find a factor binding Pit-1 binding elements in the PRL gene other than Pit-1. Beside colonies containing Pit-1 or Oct-1 cDNA, three colonies contained mPOU cDNA, a member of the POU protein family. Immunohistochemical analysis showed mPOU-like immunoreactivity was present in human PRL-producing pituitary tumors but not in non-functioning pituitary tumors. Mobility shift analysis revealed that mPOU bound to Pit-1 binding elements of the PRL gene, 1P and 3P. mPOU activated the expression of 0.6 k PRL and 7x1P reporter genes in the presence of Pit-1 and cAMP, although it did not enhance Pit-1-induced expression of 7x3P reporter gene. These findings suggest that mPOU is involved in the activation of the PRL gene by cAMP through 1P in the presence of Pit-1.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-Pyridinecarboxylic acid...,
http://linkedlifedata.com/resource/pubmed/chemical/8-((4-chlorophenyl)thio)cyclic-3',5'...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HCFC1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Host Cell Factor C1,
http://linkedlifedata.com/resource/pubmed/chemical/Octamer Transcription Factor-1,
http://linkedlifedata.com/resource/pubmed/chemical/POU Domain Factors,
http://linkedlifedata.com/resource/pubmed/chemical/POU1F1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/POU2F1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Prolactin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor Pit-1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0303-7207
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
207
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
31-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12972181-3-Pyridinecarboxylic acid...,
pubmed-meshheading:12972181-Animals,
pubmed-meshheading:12972181-Binding Sites,
pubmed-meshheading:12972181-COS Cells,
pubmed-meshheading:12972181-Calcium,
pubmed-meshheading:12972181-Cercopithecus aethiops,
pubmed-meshheading:12972181-Cloning, Molecular,
pubmed-meshheading:12972181-Cyclic AMP,
pubmed-meshheading:12972181-DNA, Complementary,
pubmed-meshheading:12972181-DNA-Binding Proteins,
pubmed-meshheading:12972181-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:12972181-Gene Expression Regulation,
pubmed-meshheading:12972181-Gene Library,
pubmed-meshheading:12972181-Genes, Reporter,
pubmed-meshheading:12972181-Genetic Vectors,
pubmed-meshheading:12972181-Host Cell Factor C1,
pubmed-meshheading:12972181-Humans,
pubmed-meshheading:12972181-Immunohistochemistry,
pubmed-meshheading:12972181-Mutation,
pubmed-meshheading:12972181-Octamer Transcription Factor-1,
pubmed-meshheading:12972181-POU Domain Factors,
pubmed-meshheading:12972181-Pituitary Gland,
pubmed-meshheading:12972181-Prolactin,
pubmed-meshheading:12972181-Promoter Regions, Genetic,
pubmed-meshheading:12972181-Protein Binding,
pubmed-meshheading:12972181-Recombinant Fusion Proteins,
pubmed-meshheading:12972181-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:12972181-Sequence Alignment,
pubmed-meshheading:12972181-Sequence Analysis, DNA,
pubmed-meshheading:12972181-Thionucleotides,
pubmed-meshheading:12972181-Transcription Factor Pit-1,
pubmed-meshheading:12972181-Transcription Factors,
pubmed-meshheading:12972181-Transfection,
pubmed-meshheading:12972181-Two-Hybrid System Techniques,
pubmed-meshheading:12972181-Yeasts
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pubmed:year |
2003
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pubmed:articleTitle |
Cloning of a protein binding to the most proximal Pit-1 binding element of prolactin gene from human pituitary cDNA library.
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pubmed:affiliation |
Division of Endocrinology/Metabolism, Neurology and Hematology/Oncology, Department of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, Kobe, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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