|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1-2
|
|
pubmed:dateCreated |
2003-9-12
|
|
pubmed:abstractText |
We have shown that measurement of mRNA for cytotoxic attack proteins perforin and granzyme B in urinary cells is a noninvasive means of diagnosing acute rejection of human renal allografts. Urinary cell mRNA studies have yielded useful information in other patient populations such as patients with cancer. The isolation of sufficient and high quality ribonucleic acid (RNA) from urinary cells however is problematic. RNAlater, an RNA stabilization solution, has been reported to optimize RNA isolation from tumor tissues stored at room temperature and from pigment-rich ocular tissues.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0022-1759
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
279
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
135-42
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:12969554-Adolescent,
pubmed-meshheading:12969554-Child,
pubmed-meshheading:12969554-Granzymes,
pubmed-meshheading:12969554-Humans,
pubmed-meshheading:12969554-RNA,
pubmed-meshheading:12969554-RNA, Ribosomal, 18S,
pubmed-meshheading:12969554-Serine Endopeptidases,
pubmed-meshheading:12969554-Spectrophotometry,
pubmed-meshheading:12969554-Transforming Growth Factor beta,
pubmed-meshheading:12969554-Urine
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Optimization of RNA yield, purity and mRNA copy number by treatment of urine cell pellets with RNAlater.
|
|
pubmed:affiliation |
Weill Medical College of Cornell University, New York, NY, USA.
|
|
pubmed:publicationType |
Journal Article
|
