|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2003-9-11
|
|
pubmed:abstractText |
(1) Little is known about the interaction of 17beta-estradiol (E2) and the vasoactive endothelin system in the heart. Endothelin signaling is activated in a failing heart and may contribute to myocardial dysfunction and remodeling. Therefore, we investigated the regulation of proteins of the endothelin system (ppET-1, ECE and ETA-R and ETB-R) in the hearts of female spontaneously hypertensive rats (SHR) with respect to E2. (2) Relative expression levels of the respective cardiac mRNA obtained from sham-operated, ovariectomized and ovariectomized E2-substituted SHR were quantified by real-time PCR. Ovariectomy led to a significant upregulation of the ETB-R mRNA (2.6+/-0.8-fold) in the left ventricular myocardium, which was not attendant with an alteration of ETA-R, ECE and ppET-1 mRNA expression. (3) An upregulation of the relative expression level of ETB-R protein due to ovariectomy was also demonstrated by radioligand binding assay. (4) Upregulation of both ETB-R mRNA and ETB-R protein expression was completely inhibited by E2 replacement. (5) To confirm these results in in vitro experiments, we quantified the mRNA of ET-R subtypes from isolated cardiomyocytes in the presence and absence of E2 (10-8 m, 24 h). Our data showed a markedly downregulated level of ETB-R mRNA in cardiomyocytes stimulated with E2. ETB-R downregulation was not attendant with the alteration of ETA-R, ECE and ppET-1 mRNA expression. (6) Taken together, these data demonstrate that estrogen regulates the expression of ETB-R in rat ventricular myocardium in vivo and in vitro. These observations may help to understand gender-based differences found in cardiovascular disease.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-10199843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-10431821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-10470989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-10690338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-10749889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-10912468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-11113050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-11583108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-11787467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-12099722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-12370225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-2054934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-2649896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-2902806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-7679333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-8022411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-8062389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-8147891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-8352786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-8371713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-8466520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-8576947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-91593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-9631868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12967949-9790900
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0007-1188
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
140
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
195-201
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:12967949-Animals,
pubmed-meshheading:12967949-Dose-Response Relationship, Drug,
pubmed-meshheading:12967949-Endothelin-1,
pubmed-meshheading:12967949-Estradiol,
pubmed-meshheading:12967949-Female,
pubmed-meshheading:12967949-Gene Expression Regulation,
pubmed-meshheading:12967949-Hypertension,
pubmed-meshheading:12967949-Myocytes, Cardiac,
pubmed-meshheading:12967949-Ovariectomy,
pubmed-meshheading:12967949-Protein Binding,
pubmed-meshheading:12967949-Rats,
pubmed-meshheading:12967949-Rats, Inbred SHR,
pubmed-meshheading:12967949-Receptor, Endothelin B
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
17 Beta-estradiol regulates the expression of endothelin receptor type B in the heart.
|
|
pubmed:affiliation |
Institut für Physiologie II, Wilhelmstr. 35-37, Universitatsklinikum Bonn 53111, Germany.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
