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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2003-9-11
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pubmed:abstractText |
Spontaneous pre-B lymphomas in SL/Kh mice occur by somatic acquisition of a provirus genome of endogenous murine leukemia virus (MuLV). Inverse PCR amplification and sequence analyses of a provirus and its host flanking fragment revealed a proviral insertion into c-myc in 3 out of 60 SL/Kh pre-B lymphomas, named Svi3 lymphomas (SL/Kh virus integration site-3). Southern blot analysis revealed that two lymphomas had clonal integration in c-myc exon 1 and the other, in the promoter region. In 2 out of 3 Svi3 lymphomas, a fusion transcript of provirus 3' long terminal repeat and c-myc and a normal full-length c-myc transcript were obtained, but in one Svi3 lymphoma, only the normal transcript was obtained. All three Svi3 lymphomas had increased c-myc expression, producing normal 67-kDa c-Myc protein. Svi3 lymphomas had more mature phenotypes in the steps of early B-cell differentiation than Svi1 lymphomas, in which c-myc expression was indirectly up-regulated by provirus integration into Stat5a.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1347-9032
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
791-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12967477-3' Untranslated Regions,
pubmed-meshheading:12967477-Animals,
pubmed-meshheading:12967477-Blotting, Northern,
pubmed-meshheading:12967477-Blotting, Southern,
pubmed-meshheading:12967477-DNA, Neoplasm,
pubmed-meshheading:12967477-DNA Primers,
pubmed-meshheading:12967477-DNA-Binding Proteins,
pubmed-meshheading:12967477-Exons,
pubmed-meshheading:12967477-Genes, myc,
pubmed-meshheading:12967477-Introns,
pubmed-meshheading:12967477-Leukemia Virus, Murine,
pubmed-meshheading:12967477-Lymphoma, B-Cell,
pubmed-meshheading:12967477-Mice,
pubmed-meshheading:12967477-Mice, Inbred Strains,
pubmed-meshheading:12967477-Milk Proteins,
pubmed-meshheading:12967477-NIH 3T3 Cells,
pubmed-meshheading:12967477-Phenotype,
pubmed-meshheading:12967477-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12967477-STAT5 Transcription Factor,
pubmed-meshheading:12967477-Trans-Activators,
pubmed-meshheading:12967477-Transfection,
pubmed-meshheading:12967477-Virus Integration
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pubmed:year |
2003
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pubmed:articleTitle |
Svi3: A provirus common integration site in c-myc in SL/Kh pre-B lymphomas.
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pubmed:affiliation |
Department of Pathology and Biology of Diseases, Kyoto University Graduate School of Medicine, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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