Linked Life Data

1296671

Source:http://linkedlifedata.com/resource/pubmed/id/1296671

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-4
pubmed:dateCreated
1993-4-28
pubmed:abstractText
Although abundant, available information regarding the values of circulating thyronines in the rainbow trout is misleading and confusing. The present study demonstrates the importance of maintaining species serum-homology when measuring thyronines with RIA to avoid spurious results. The utilization of homologous (trout) serum free of thyronines in the standard curve allowed the standardization of accurate and specific radioimmunoassays to quantitate circulating T3 and T4 levels in 10 microliters of rainbow trout serum. Detection limit was 50 pg for both thyronines. Inter- and intra-assay coefficients of variation were minor to 10%. Mean values for T3 and T4 +/- SEM in the trout population studied (n = 34) were 23.6 +/- 1.2 and 12.0 +/- 2.3 ng/ml respectively. A positive and significant correlation (p < 0.005) between body weight and T3 was observed, confirming the active participation of this thyronine in growth processes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0067-9666
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
41-7
pubmed:dateRevised
2009-11-11
pubmed:meshHeading
pubmed:articleTitle
The importance of employing homologous serum free of thyronines in radioimmunoassays to assess circulating thyroid hormones in rainbow trout.
pubmed:affiliation
Departamento de Fisiología, Universidad Nacional Autónoma de México, D.F.
pubmed:publicationType
Journal Article