| pubmed-article:12965933 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12965933 | lifeskim:mentions | umls-concept:C1522642 | lld:lifeskim |
| pubmed-article:12965933 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:12965933 | lifeskim:mentions | umls-concept:C0205470 | lld:lifeskim |
| pubmed-article:12965933 | lifeskim:mentions | umls-concept:C0027321 | lld:lifeskim |
| pubmed-article:12965933 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:12965933 | lifeskim:mentions | umls-concept:C0450254 | lld:lifeskim |
| pubmed-article:12965933 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:12965933 | pubmed:dateCreated | 2003-9-10 | lld:pubmed |
| pubmed-article:12965933 | pubmed:abstractText | We previously cloned an antigenic gene (named nfa1) from a cDNA library of Naegleria fowleri by immunoscreening. The nfa1 gene had a coding nucleotide sequence consisting of 357 bases and produced a recombinant 13.1-kDa protein (Nfa1). In this study, to get more information regarding the recombinant Nfa1 protein (rNfa1), we produced an anti-Nfa1 polyclonal antibody from mice immunized with rNfa1 and used a peroxidase staining method to carry out immunocytochemistry experiments. In addition, we observed the effect of the presence of an anti-Nfa1 antibody on the in vitro cytotoxicity of N. fowleri against Chinese hamster ovary (CHO) cells. Trophozoites of N. fowleri in cultivation reacted strongly with a peroxidase-labeled anti-Nfa1 antibody. In inflammatory and necrotic regions of brain tissue infected with N. fowleri, labeled trophozoites that were stained brown were also observed. When examined using a transmission electron microscope, the Nfa1 protein showed pseudopodium-specific immunolocalization on a trophozoite of N. fowleri. When examined using a light microscope, CHO cells grown in cocultures with N. fowleri trophozoites (group I) for 48 h showed morphologically severe destruction but CHO cells grown in cocultures with N. fowleri trophozoites and an anti-Nfa1 polyclonal antibody (group II) showed less destruction. The results of a lactate dehydrogenase release assay showed that group I CHO cells exhibited 81% cytotoxicity and group II CHO cells exhibited 13.8% cytotoxicity. | lld:pubmed |
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| pubmed-article:12965933 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12965933 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12965933 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:12965933 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12965933 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:12965933 | pubmed:issn | 1071-412X | lld:pubmed |
| pubmed-article:12965933 | pubmed:author | pubmed-author:ParkSunS | lld:pubmed |
| pubmed-article:12965933 | pubmed:author | pubmed-author:SohnSeonghyan... | lld:pubmed |
| pubmed-article:12965933 | pubmed:author | pubmed-author:ImKyung-IlKI | lld:pubmed |
| pubmed-article:12965933 | pubmed:author | pubmed-author:KimHyung-IlHI | lld:pubmed |
| pubmed-article:12965933 | pubmed:author | pubmed-author:KimKyongmin... | lld:pubmed |
| pubmed-article:12965933 | pubmed:author | pubmed-author:ShinHo-JoonHJ | lld:pubmed |
| pubmed-article:12965933 | pubmed:author | pubmed-author:ChoMyoung-Soo... | lld:pubmed |
| pubmed-article:12965933 | pubmed:author | pubmed-author:JungSuk-YulSY | lld:pubmed |
| pubmed-article:12965933 | pubmed:author | pubmed-author:KimHan-JipHJ | lld:pubmed |
| pubmed-article:12965933 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12965933 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:12965933 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12965933 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12965933 | pubmed:pagination | 954-9 | lld:pubmed |
| pubmed-article:12965933 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:12965933 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12965933 | pubmed:articleTitle | Immunological characterizations of a cloned 13.1-kilodalton protein from pathogenic Naegleria fowleri. | lld:pubmed |
| pubmed-article:12965933 | pubmed:affiliation | Department of Microbiology, School of Medicine, Ajou University, Suwon 442-749, Korea. | lld:pubmed |
| pubmed-article:12965933 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12965933 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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