|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5
|
|
pubmed:dateCreated |
2003-9-10
|
|
pubmed:abstractText |
We previously cloned an antigenic gene (named nfa1) from a cDNA library of Naegleria fowleri by immunoscreening. The nfa1 gene had a coding nucleotide sequence consisting of 357 bases and produced a recombinant 13.1-kDa protein (Nfa1). In this study, to get more information regarding the recombinant Nfa1 protein (rNfa1), we produced an anti-Nfa1 polyclonal antibody from mice immunized with rNfa1 and used a peroxidase staining method to carry out immunocytochemistry experiments. In addition, we observed the effect of the presence of an anti-Nfa1 antibody on the in vitro cytotoxicity of N. fowleri against Chinese hamster ovary (CHO) cells. Trophozoites of N. fowleri in cultivation reacted strongly with a peroxidase-labeled anti-Nfa1 antibody. In inflammatory and necrotic regions of brain tissue infected with N. fowleri, labeled trophozoites that were stained brown were also observed. When examined using a transmission electron microscope, the Nfa1 protein showed pseudopodium-specific immunolocalization on a trophozoite of N. fowleri. When examined using a light microscope, CHO cells grown in cocultures with N. fowleri trophozoites (group I) for 48 h showed morphologically severe destruction but CHO cells grown in cocultures with N. fowleri trophozoites and an anti-Nfa1 polyclonal antibody (group II) showed less destruction. The results of a lactate dehydrogenase release assay showed that group I CHO cells exhibited 81% cytotoxicity and group II CHO cells exhibited 13.8% cytotoxicity.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12965933-11831780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12965933-1319405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12965933-2193354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12965933-2437151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12965933-3308948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12965933-3959003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12965933-4989229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12965933-5005026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12965933-5145006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12965933-6853686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12965933-6855619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12965933-7216418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12965933-8458963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12965933-8627505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12965933-9034567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12965933-942051
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
1071-412X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
10
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
954-9
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:12965933-Amebiasis,
pubmed-meshheading:12965933-Animals,
pubmed-meshheading:12965933-Antibodies, Protozoan,
pubmed-meshheading:12965933-Antigens, Protozoan,
pubmed-meshheading:12965933-Blotting, Western,
pubmed-meshheading:12965933-Brain,
pubmed-meshheading:12965933-CHO Cells,
pubmed-meshheading:12965933-Cloning, Molecular,
pubmed-meshheading:12965933-Coculture Techniques,
pubmed-meshheading:12965933-Cricetinae,
pubmed-meshheading:12965933-Cytotoxicity, Immunologic,
pubmed-meshheading:12965933-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:12965933-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12965933-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:12965933-Female,
pubmed-meshheading:12965933-Immunohistochemistry,
pubmed-meshheading:12965933-Mice,
pubmed-meshheading:12965933-Microscopy, Electron,
pubmed-meshheading:12965933-Naegleria fowleri,
pubmed-meshheading:12965933-Polymerase Chain Reaction,
pubmed-meshheading:12965933-Protozoan Proteins,
pubmed-meshheading:12965933-Recombinant Proteins
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Immunological characterizations of a cloned 13.1-kilodalton protein from pathogenic Naegleria fowleri.
|
|
pubmed:affiliation |
Department of Microbiology, School of Medicine, Ajou University, Suwon 442-749, Korea.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
