Linked Life Data

12963086

Source:http://linkedlifedata.com/resource/pubmed/id/12963086

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-9-9
pubmed:abstractText
Munc18-1 (p67, nSec1, rbSec1), a neuron-specific 67kDa protein was independently identified as a syntaxin-binding protein, and as a component that co-purifies with, and regulates the kinase activity of cyclin dependent kinase (Cdk5). Gene knockout studies have demonstrated a role for Munc18-1 in synaptic vesicle docking and neurotransmitter release. Mice lacking Munc18-1 gene were synaptically silent, but the gene deletion did not prevent normal brain assembly, including the formation of layered structures, fiber pathways and morphologically defined synapses. Previous study has shown that Munc18-1 facilitates Cdk5 mediated phosphorylation of KSPXK domains of the neuronal cytoskeletal elements, suggesting that Munc18-1 may function in the regulation of cytoskeletal dynamics. Present study demonstrates the co-purification and co-localization of Munc18 with cytoskeletal elements and forms first step towards understanding the role for Munc18-1 in cytoskeletal dynamics. Conversely, the cytoskeletal proteins and Cdk5 co-purifies with Munc18-1 in a Munc18-1 immuno-affinity chromatography, suggesting a strong protein-protein interaction. Findings from immunofluorescence studies in PC12 cells have shown co-localization of Munc18-1 and Cdk5 with neurofilaments and microtubules. Further, immunohistochemical and immuno-electron microscopic studies of rat olfactory bulb also demonstrated co-localization of Munc18-1 and Cdk5 with cytoskeletal elements. Thus, the biochemical evidence of strong interaction between Munc18-1 with cytoskeletal proteins and morphological evidence of their (Munc18 and cytoskeletal elements) identical sub-cellular localization is suggestive of the possible role for Munc18-1 in cytoskeletal dynamics.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0197-0186
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-44
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:12963086-Animals, pubmed-meshheading:12963086-Blotting, Western, pubmed-meshheading:12963086-Cyclin-Dependent Kinase 5, pubmed-meshheading:12963086-Cyclin-Dependent Kinases, pubmed-meshheading:12963086-Cytoskeletal Proteins, pubmed-meshheading:12963086-Fluorescent Antibody Technique, pubmed-meshheading:12963086-Immunochemistry, pubmed-meshheading:12963086-Immunohistochemistry, pubmed-meshheading:12963086-Male, pubmed-meshheading:12963086-Microscopy, Immunoelectron, pubmed-meshheading:12963086-Munc18 Proteins, pubmed-meshheading:12963086-Nerve Tissue Proteins, pubmed-meshheading:12963086-Neurons, pubmed-meshheading:12963086-Olfactory Bulb, pubmed-meshheading:12963086-PC12 Cells, pubmed-meshheading:12963086-Proteins, pubmed-meshheading:12963086-Rats, pubmed-meshheading:12963086-Rats, Wistar, pubmed-meshheading:12963086-Solubility, pubmed-meshheading:12963086-Vesicular Transport Proteins
pubmed:year
2004
pubmed:articleTitle
Co-purification and localization of Munc18-1 (p67) and Cdk5 with neuronal cytoskeletal proteins.
pubmed:affiliation
Department of Neuropathology, National Institute of Mental Health and Neurosciences, Bangalore 560029, India.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't