Source:http://linkedlifedata.com/resource/pubmed/id/12959933
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2003-12-5
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| pubmed:abstractText |
Bombesin-peptide (BLP) immunoreactivity occurs at high levels in fetal lung. Previous studies showed that bombesin promotes fetal lung development. To test the hypothesis that such effects are mediated by known mammalian bombesin receptors [gastrin-releasing peptide (GRP)/bombesin-preferring receptor (GRPR), neuromedin B (NMB) receptor (NMBR), and the orphan bombesin receptor subtype-3 (BRS-3)], we analyzed the ontogeny of GRPR, NMBR, and BRS-3 gene expression in mouse lung. We examined the regulation of these three genes by dexamethasone and bombesin, which modulate lung development. Using incorporation of [3H]thymidine and [3H]choline, we then assessed whether GRP, NMB, and Leu8-phyllolitorin modulate lung growth and maturation in fetal lung explants. GRPR gene expression was detected predominantly in utero, whereas NMBR and BRS-3 genes were expressed from embryonic days 13-16 and on multiple postnatal days. All three mRNAs are present in airway epithelium and mesenchymal cells but occur in different relative patterns. These genes were regulated differently. Dexamethasone and bombesin increased GRPR mRNA, bombesin downregulated NMBR, and neither agent affected BRS-3. GRP increased incorporation of [3H]thymidine and [3H]choline in explants, whereas NMB induced cell proliferation and Leu8-phyllolitorin yielded variable results. Cumulative data suggest the involvement of multiple BLP receptors, including novel molecules, and argue against simple functional redundancy within this gene family during lung development.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bombesin,
http://linkedlifedata.com/resource/pubmed/chemical/Choline,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Glucocorticoids,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Bombesin,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium,
http://linkedlifedata.com/resource/pubmed/chemical/bombesin receptor subtype 3
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1040-0605
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
286
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
L165-73
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:12959933-Amino Acid Sequence,
pubmed-meshheading:12959933-Animals,
pubmed-meshheading:12959933-Bombesin,
pubmed-meshheading:12959933-Cell Differentiation,
pubmed-meshheading:12959933-Cell Division,
pubmed-meshheading:12959933-Choline,
pubmed-meshheading:12959933-Dexamethasone,
pubmed-meshheading:12959933-Female,
pubmed-meshheading:12959933-Gene Expression Regulation, Developmental,
pubmed-meshheading:12959933-Glucocorticoids,
pubmed-meshheading:12959933-In Situ Hybridization,
pubmed-meshheading:12959933-Lung,
pubmed-meshheading:12959933-Mice,
pubmed-meshheading:12959933-Molecular Sequence Data,
pubmed-meshheading:12959933-Organ Culture Techniques,
pubmed-meshheading:12959933-Pregnancy,
pubmed-meshheading:12959933-RNA, Messenger,
pubmed-meshheading:12959933-Receptors, Bombesin,
pubmed-meshheading:12959933-Thymidine,
pubmed-meshheading:12959933-Tritium
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| pubmed:year |
2004
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| pubmed:articleTitle |
Bombesin-like peptide receptor gene expression, regulation, and function in fetal murine lung.
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| pubmed:affiliation |
Department of Pathology, Children's and Brigham and Women's Hospitals and Harvard Medical School, Boston, MA 02115, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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