Linked Life Data

12959573

Source:http://linkedlifedata.com/resource/pubmed/id/12959573

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2003-9-8
pubmed:abstractText
It is becoming clearer that genetic activity is closely associated with the intracellular energy state. However, the mechanisms of this association are still unclear. In this study, we focused on large-scale changes in the structure of DNA to examine the effect of the NTP concentration on the transcription reaction with T7 RNA polymerase and compared the results with long duplex DNA to those with a short persistent-length(1) fragment. The transcriptional activity dramatically changed only for long duplex DNA within a narrow range of NTP concentrations associated with changes in the large-scale structure of DNA. This result suggests that the energy state may play an essential role in regulating ON/OFF switching on transcriptional activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1525-7797
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1121-5
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:articleTitle
NTP concentration switches transcriptional activity by changing the large-scale structure of DNA.
pubmed:affiliation
Department of Physics, Graduate School of Science, Kyoto University, and CREST, Kyoto 606-8502, Japan.
pubmed:publicationType
Journal Article