Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2003-9-5
pubmed:abstractText
The pre-transformed human fetal brain cDNA library was used to screen the protein interacting with neuroglobin by using yeast two hybrid system III from ClonTech Inc. The protein encoded by one of the clones interacting with neuroglobin (NGB) was confirmed to be the C terminus of the Na(+), K(+)-ATPase beta2 subunit (NKA1b2) based on amino acid sequences. Then the full-length coding region cDNA sequence of NKA1b2 was obtained from human fetal brain cDNA library by PCR. A set of experiments were designed to test the interaction between NGB and NKA1b2. Interaction between NGB and NKA1b2 was confirmed by binding assay in vitro. Furthermore, the interaction was also proved by co-immunoprecipitation test in vivo. Moreover, the structure integrity of neuroglobin was found to be essential for the interaction between NGB and NKA1b2 by yeast two hybrid method with a series of neuroglobin truncated mutants.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0582-9879
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
823-8
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:12958655-Amino Acid Sequence, pubmed-meshheading:12958655-Animals, pubmed-meshheading:12958655-Binding Sites, pubmed-meshheading:12958655-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:12958655-Globins, pubmed-meshheading:12958655-Glutathione Transferase, pubmed-meshheading:12958655-Humans, pubmed-meshheading:12958655-Mice, pubmed-meshheading:12958655-Molecular Sequence Data, pubmed-meshheading:12958655-Nerve Tissue Proteins, pubmed-meshheading:12958655-Protein Binding, pubmed-meshheading:12958655-Protein Subunits, pubmed-meshheading:12958655-Recombinant Fusion Proteins, pubmed-meshheading:12958655-Saccharomyces cerevisiae, pubmed-meshheading:12958655-Sequence Homology, Amino Acid, pubmed-meshheading:12958655-Sodium-Potassium-Exchanging ATPase, pubmed-meshheading:12958655-Two-Hybrid System Techniques
pubmed:year
2003
pubmed:articleTitle
[Identification of interaction and interaction domains between neuroglobin and Na(+), K(+)-ATPase beta2 subunit].
pubmed:affiliation
Beijing Institute of Radiation Medicine, Beijing 100850, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't