Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2003-9-5
pubmed:abstractText
Mycobacterium tuberculosis and Mycobacterium avium are pathogenic slow-growing mycobacteria that cause distinct human diseases. In contrast to recent advances in M. tuberculosis genetics and pathogenesis investigation, M. avium has remained genetically intractable and, consequently, its pathogenic strategies remain poorly understood. Here we report the successful development of efficient allelic exchange and transposon mutagenesis in an opaque clinical strain of M. avium by specialized transduction. Efforts to disrupt the leuD gene of M. avium by specialized transduction were successful but were complicated by inefficient isolation of recombinants secondary to high spontaneous antibiotic resistance. However, by using this leucine auxotroph as a genetic host and the Streptomyces coelicolor leuD gene as a selectable marker, we achieved efficient allelic exchange at the M. avium pcaA locus. A leuD-marked transposon delivered by specialized transduction mutagenized M. avium with efficiencies similar to M. tuberculosis. These results establish a system for random and directed mutagenesis of M. avium. In combination with the forthcoming M. avium genome sequence, these tools will allow the distinct physiologic and pathogenic properties of M. avium to be dissected in molecular detail.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-10361705, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-10438745, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-10564470, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-10573420, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-10768986, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-10882107, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-11092877, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-11682502, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-11849545, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-12000953, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-12368431, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-12368434, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-12370991, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-7868221, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-7955524, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-8047000, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-8303277, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-8613393, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-8809745, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-8844653, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-9380741, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-9399525, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-9673242, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-9767568, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-9784137, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-9817824, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-9921481, http://linkedlifedata.com/resource/pubmed/commentcorrection/12957884-9921495
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5039-44
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Efficient allelic exchange and transposon mutagenesis in Mycobacterium avium by specialized transduction.
pubmed:affiliation
Howard Hughes Medical Institute, Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.