| pubmed-article:12957399 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12957399 | lifeskim:mentions | umls-concept:C0027651 | lld:lifeskim |
| pubmed-article:12957399 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:12957399 | lifeskim:mentions | umls-concept:C0032098 | lld:lifeskim |
| pubmed-article:12957399 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:12957399 | lifeskim:mentions | umls-concept:C0042210 | lld:lifeskim |
| pubmed-article:12957399 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:12957399 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:12957399 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:12957399 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:12957399 | lifeskim:mentions | umls-concept:C1456348 | lld:lifeskim |
| pubmed-article:12957399 | lifeskim:mentions | umls-concept:C1519885 | lld:lifeskim |
| pubmed-article:12957399 | lifeskim:mentions | umls-concept:C1881197 | lld:lifeskim |
| pubmed-article:12957399 | lifeskim:mentions | umls-concept:C2347946 | lld:lifeskim |
| pubmed-article:12957399 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:12957399 | pubmed:dateCreated | 2003-9-5 | lld:pubmed |
| pubmed-article:12957399 | pubmed:abstractText | We have developed a method for rapidly producing in plants the idiotype regions of the tumor-specific Ig as single-chain Fv (scFv) proteins for use in the treatment of non-Hodgkin's lymphoma. Variable region gene sequences were generated from either a tumor hybridoma or human tumor biopsy cells, and idiotype domains were joined by a novel linker and cloned into a modified tobacco mosaic virus (TMV) vector designed to secrete the scFv protein in infected Nicotiana benthamiana plants. Thirty-eight out of 44 human scFv proteins showed Coomassie visible material in crude secretory (interstitial fluid, IF) extracts, 21 of those between 100 and 800 microg/ml. Eight of these proteins were tested for appropriate idiotype responses in vaccinated mice. In all eight cases, anti-idiotype immune responses were induced with minimal cross reactivity to irrelevant Ig or scFv proteins. Four out of four anti-scFv sera were also shown to recognize the Ig on human tumor cells by flow cytometry analysis. | lld:pubmed |
| pubmed-article:12957399 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12957399 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12957399 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12957399 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12957399 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12957399 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12957399 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:12957399 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12957399 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12957399 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12957399 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:12957399 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:12957399 | pubmed:author | pubmed-author:LevyRonaldR | lld:pubmed |
| pubmed-article:12957399 | pubmed:author | pubmed-author:McCormickAlis... | lld:pubmed |
| pubmed-article:12957399 | pubmed:author | pubmed-author:ReinlStephen... | lld:pubmed |
| pubmed-article:12957399 | pubmed:author | pubmed-author:CameronTerri... | lld:pubmed |
| pubmed-article:12957399 | pubmed:author | pubmed-author:VojdaniFakhri... | lld:pubmed |
| pubmed-article:12957399 | pubmed:author | pubmed-author:FronefieldMic... | lld:pubmed |
| pubmed-article:12957399 | pubmed:author | pubmed-author:TuséDanielD | lld:pubmed |
| pubmed-article:12957399 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12957399 | pubmed:volume | 278 | lld:pubmed |
| pubmed-article:12957399 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12957399 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12957399 | pubmed:pagination | 95-104 | lld:pubmed |
| pubmed-article:12957399 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:12957399 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12957399 | pubmed:articleTitle | Individualized human scFv vaccines produced in plants: humoral anti-idiotype responses in vaccinated mice confirm relevance to the tumor Ig. | lld:pubmed |
| pubmed-article:12957399 | pubmed:affiliation | Large Scale Biology Corporation, Vacaville, CA 95688, USA. alison.mccormick@lsbc.com | lld:pubmed |
| pubmed-article:12957399 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12957399 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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