|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0026809,
umls-concept:C0027651,
umls-concept:C0032098,
umls-concept:C0042210,
umls-concept:C0086418,
umls-concept:C0871261,
umls-concept:C1456348,
umls-concept:C1519885,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1881197,
umls-concept:C2347946,
umls-concept:C2911692
|
|
pubmed:issue |
1-2
|
|
pubmed:dateCreated |
2003-9-5
|
|
pubmed:abstractText |
We have developed a method for rapidly producing in plants the idiotype regions of the tumor-specific Ig as single-chain Fv (scFv) proteins for use in the treatment of non-Hodgkin's lymphoma. Variable region gene sequences were generated from either a tumor hybridoma or human tumor biopsy cells, and idiotype domains were joined by a novel linker and cloned into a modified tobacco mosaic virus (TMV) vector designed to secrete the scFv protein in infected Nicotiana benthamiana plants. Thirty-eight out of 44 human scFv proteins showed Coomassie visible material in crude secretory (interstitial fluid, IF) extracts, 21 of those between 100 and 800 microg/ml. Eight of these proteins were tested for appropriate idiotype responses in vaccinated mice. In all eight cases, anti-idiotype immune responses were induced with minimal cross reactivity to irrelevant Ig or scFv proteins. Four out of four anti-scFv sera were also shown to recognize the Ig on human tumor cells by flow cytometry analysis.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Anti-Idiotypic,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, B-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0022-1759
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
278
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
95-104
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:12957399-Animals,
pubmed-meshheading:12957399-Antibodies, Anti-Idiotypic,
pubmed-meshheading:12957399-Antigens, Neoplasm,
pubmed-meshheading:12957399-Blotting, Western,
pubmed-meshheading:12957399-Cloning, Molecular,
pubmed-meshheading:12957399-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12957399-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:12957399-Flow Cytometry,
pubmed-meshheading:12957399-Gene Library,
pubmed-meshheading:12957399-Genetic Vectors,
pubmed-meshheading:12957399-Humans,
pubmed-meshheading:12957399-Hybridomas,
pubmed-meshheading:12957399-Immunoglobulin Variable Region,
pubmed-meshheading:12957399-Lymphoma, Non-Hodgkin,
pubmed-meshheading:12957399-Mice,
pubmed-meshheading:12957399-Polymerase Chain Reaction,
pubmed-meshheading:12957399-Receptors, Antigen, B-Cell,
pubmed-meshheading:12957399-Tobacco,
pubmed-meshheading:12957399-Tobacco Mosaic Virus,
pubmed-meshheading:12957399-Tumor Cells, Cultured,
pubmed-meshheading:12957399-Vaccines,
pubmed-meshheading:12957399-Vaccines, Synthetic
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Individualized human scFv vaccines produced in plants: humoral anti-idiotype responses in vaccinated mice confirm relevance to the tumor Ig.
|
|
pubmed:affiliation |
Large Scale Biology Corporation, Vacaville, CA 95688, USA. alison.mccormick@lsbc.com
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
