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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2003-9-5
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pubmed:abstractText |
We have targeted a K-ras allele in mouse embryonic stem (ES) cells to express a K-Ras(V12) oncoprotein along with a marker protein (beta-geo) from a single bicistronic transcript. Expression of this oncogenic allele requires removal of a knocked in STOP transcriptional cassette by Cre recombinase. Primary mouse embryonic fibroblasts expressing this K-ras(V12) allele do not undergo proliferative senescence and proliferate as immortal cells. In mice, expression of K-ras(V12) throughout the body fails to induce unscheduled proliferation or other growth abnormalities for up to eight months. Only a percentage of K-ras(V12)-expressing lung bronchiolo-alveolar cells undergo malignant transformation leading to the formation of multiple adenomas and adenocarcinomas. These results indicate that neoplastic growth induced by an endogenous K-ras oncogene depends upon cellular context.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1535-6108
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
111-20
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12957286-Animals,
pubmed-meshheading:12957286-Cell Aging,
pubmed-meshheading:12957286-Cell Division,
pubmed-meshheading:12957286-Cell Line, Transformed,
pubmed-meshheading:12957286-Cell Transformation, Neoplastic,
pubmed-meshheading:12957286-Chromosome Aberrations,
pubmed-meshheading:12957286-Cyclin-Dependent Kinase Inhibitor p16,
pubmed-meshheading:12957286-Fibroblasts,
pubmed-meshheading:12957286-Gene Targeting,
pubmed-meshheading:12957286-Genes, ras,
pubmed-meshheading:12957286-Genetic Vectors,
pubmed-meshheading:12957286-MAP Kinase Signaling System,
pubmed-meshheading:12957286-Mice,
pubmed-meshheading:12957286-Mice, Transgenic,
pubmed-meshheading:12957286-Neoplasms,
pubmed-meshheading:12957286-Oncogene Protein p21(ras),
pubmed-meshheading:12957286-Protein-Serine-Threonine Kinases,
pubmed-meshheading:12957286-Proto-Oncogene Proteins,
pubmed-meshheading:12957286-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:12957286-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12957286-Stem Cells,
pubmed-meshheading:12957286-Survival Rate,
pubmed-meshheading:12957286-Tumor Suppressor Protein p14ARF,
pubmed-meshheading:12957286-Tumor Suppressor Protein p53
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pubmed:year |
2003
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pubmed:articleTitle |
Tumor induction by an endogenous K-ras oncogene is highly dependent on cellular context.
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pubmed:affiliation |
Molecular Oncology Programme, Centro Nacional de Investigaciones Oncológicas (CNIO), Melchor Fernández Almagro 3, 28029, Madrid, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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