Linked Life Data

12957177

Source:http://linkedlifedata.com/resource/pubmed/id/12957177

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-9-5
pubmed:abstractText
A capillary electrophoresis (CE) method has been developed and optimized for the detection of Gly-Pro dipeptide in complex biological samples: medium, cell layer and matrix obtained from long term cultured human fibroblasts of control and prolidase deficiency patients. The influence of different detergents in the sample preparation and electrophoretic conditions were investigated. The method was validated for cellular extracts with respect to limits of detection and quantitation, precision, linearity, accuracy and robustness. The optimized method was applied to real samples and revealed a significant increase of intracellular Gly-Pro dipeptide in prolidase deficiency fibroblasts with respect to the control.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1570-0232
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
795
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
133-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Optimization of a capillary electrophoretic method to detect and quantify the Gly-Pro dipeptide in complex matrices from long term cultured prolidase deficiency fibroblasts.
pubmed:affiliation
Dipartimento di Biochimica "A. Castellani", University of Pavia, 27100 Pavia, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't