| pubmed-article:12952084 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12952084 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:12952084 | lifeskim:mentions | umls-concept:C0683145 | lld:lifeskim |
| pubmed-article:12952084 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:12952084 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:12952084 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:12952084 | lifeskim:mentions | umls-concept:C1081672 | lld:lifeskim |
| pubmed-article:12952084 | pubmed:issue | 7-8 | lld:pubmed |
| pubmed-article:12952084 | pubmed:dateCreated | 2003-9-3 | lld:pubmed |
| pubmed-article:12952084 | pubmed:abstractText | A gene, zyg1, was isolated by differential screening from Dictyostelium mucoroides-7 (Dm7) cells, as one preferentially expressed during their sexual development. The zyg1 gene encodes for a novel protein (ZYG1; deduced Mr 29.4 x 10(3)) consisting of 268 amino acids. Although the ZYG1 protein has predicted PKC phosphorylation sites, it has neither transmembrane domains nor specified signal sequences. The expression of zyg1 was initiated after 2 h of starvation and reached its maximum level at 8 h under submerged conditions. The expression pattern is quite similar to the temporal change of zygote formation during sexual development (macrocyst formation) with 1 h of precedence. The zyg1 mRNA in Dm7 cells developing on agar was retained until zygotes were formed. Zyg1-overexpressing cells derived from Dm7 cells eventually formed many loose mounds, in which giant cells were surrounded by normal-sized cells, in addition to mature macrocysts even under the conditions favouring for asexual sorocarp formation. Giant cells were found by DAPI-staining to be multinucleate, possibly because of the precocious overexpression of zyg1 mRNA. Western blottings using a specific antibody raised against the oligopeptide near the C-terminal region of ZYG1 also showed that ZYG1 was actually over-produced in the zyg1-overexpressing cells. From these results, it is evident that the zyg1 gene has an essential role in zygote formation by inducing sexual cell fusion. | lld:pubmed |
| pubmed-article:12952084 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12952084 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12952084 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12952084 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12952084 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12952084 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12952084 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12952084 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12952084 | pubmed:issn | 0142-4319 | lld:pubmed |
| pubmed-article:12952084 | pubmed:author | pubmed-author:AmagaiAikoA | lld:pubmed |
| pubmed-article:12952084 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12952084 | pubmed:volume | 23 | lld:pubmed |
| pubmed-article:12952084 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12952084 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12952084 | pubmed:pagination | 867-74 | lld:pubmed |
| pubmed-article:12952084 | pubmed:dateRevised | 2010-2-12 | lld:pubmed |
| pubmed-article:12952084 | pubmed:meshHeading | pubmed-meshheading:12952084... | lld:pubmed |
| pubmed-article:12952084 | pubmed:meshHeading | pubmed-meshheading:12952084... | lld:pubmed |
| pubmed-article:12952084 | pubmed:meshHeading | pubmed-meshheading:12952084... | lld:pubmed |
| pubmed-article:12952084 | pubmed:meshHeading | pubmed-meshheading:12952084... | lld:pubmed |
| pubmed-article:12952084 | pubmed:meshHeading | pubmed-meshheading:12952084... | lld:pubmed |
| pubmed-article:12952084 | pubmed:meshHeading | pubmed-meshheading:12952084... | lld:pubmed |
| pubmed-article:12952084 | pubmed:meshHeading | pubmed-meshheading:12952084... | lld:pubmed |
| pubmed-article:12952084 | pubmed:meshHeading | pubmed-meshheading:12952084... | lld:pubmed |
| pubmed-article:12952084 | pubmed:meshHeading | pubmed-meshheading:12952084... | lld:pubmed |
| pubmed-article:12952084 | pubmed:meshHeading | pubmed-meshheading:12952084... | lld:pubmed |
| pubmed-article:12952084 | pubmed:meshHeading | pubmed-meshheading:12952084... | lld:pubmed |
| pubmed-article:12952084 | pubmed:meshHeading | pubmed-meshheading:12952084... | lld:pubmed |
| pubmed-article:12952084 | pubmed:meshHeading | pubmed-meshheading:12952084... | lld:pubmed |
| pubmed-article:12952084 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12952084 | pubmed:articleTitle | Involvement of a novel gene, zyg1, in zygote formation of Dictyostelium mucoroides. | lld:pubmed |
| pubmed-article:12952084 | pubmed:affiliation | Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, Aoba, Sendai 980-8578, Japan. aiamagai@mail.cc.tohoku.ac.jp | lld:pubmed |
| pubmed-article:12952084 | pubmed:publicationType | Journal Article | lld:pubmed |
