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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
2003-9-1
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pubmed:abstractText |
A 254-nucleotide model mRNA, designated deltaermC mRNA, was used to study the effects of translational signals and ribosome transit on mRNA decay in Bacillus subtilis. DeltaermC mRNA features a strong ribosome-binding site (RBS) and a 62-amino-acid-encoding open reading frame, followed by a transcription terminator structure. Inactivation of the RBS or the start codon resulted in a fourfold decrease in the mRNA half-life, demonstrating the importance of ternary complex formation for mRNA stability. Data for the decay of deltaermC mRNAs with stop codons at positions increasingly proximal to the translational start site showed that actual translation--even the formation of the first peptide bond--was not important for stability. The half-life of an untranslated 3.2-kb deltaermC-lacZ fusion RNA was similar to that of a translated deltaermC-lacZ mRNA, indicating that the translation of even a longer RNA was not required for wild-type stability. The data are consistent with a model in which ribosome binding and the formation of the ternary complex interfere with a 5'-end-dependent activity, possibly a 5'-binding endonuclease, which is required for the initiation of mRNA decay. This model is supported by the finding that increasing the distance from the 5' end to the start codon resulted in a 2.5-fold decrease in the mRNA half-life. These results underscore the importance of the 5' end to mRNA stability in B. subtilis.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12949089-10200269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12949089-10684583,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/12949089-9826500
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
185
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5372-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12949089-Bacillus subtilis,
pubmed-meshheading:12949089-Binding Sites,
pubmed-meshheading:12949089-Codon, Initiator,
pubmed-meshheading:12949089-Erythromycin,
pubmed-meshheading:12949089-Half-Life,
pubmed-meshheading:12949089-Methyltransferases,
pubmed-meshheading:12949089-Mutation,
pubmed-meshheading:12949089-Protein Biosynthesis,
pubmed-meshheading:12949089-RNA, Messenger,
pubmed-meshheading:12949089-RNA Stability,
pubmed-meshheading:12949089-Ribosomes,
pubmed-meshheading:12949089-Sequence Deletion
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pubmed:year |
2003
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pubmed:articleTitle |
Effect of translational signals on mRNA decay in Bacillus subtilis.
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pubmed:affiliation |
Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York University, New York, New York 10029, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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