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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1993-4-15
|
| pubmed:abstractText |
Twenty hair samples obtained from Bolivian mine workers who chewed 3-8 g of coca leaves daily for several years were analyzed for cocaine and its main metabolites, benzoylecgonine (BZE) and ecgonine methyl ester (EME). A new method was developed for the detection and quantitation of cocaine and its metabolites, BZE and EME, from hair in a single procedure. The hair samples were washed, cut into 56 segments (2-cm length), pulverized, and incubated with phosphate buffer and the enzyme beta-glucuronidase-arylsulfatase. After solid phase extraction and derivatization with pentafluoropropionic anhydride/pentafluoropropanol, the drugs were identified and measured by gas chromatography/mass spectrometry (GC/MS) using deuterated cocaine, BZE, and EME as internal standards. The method is reproducible (cocaine, CV = 8%; BZE, CV = 14%) and the detection limit for cocaine and BZE was 0.1 ng/mg, for EME 1 ng/mg. In the different hair segments, cocaine was found to be present in concentrations between 1.4 to 50.6 ng/mg, benzoylecgonine from 0.4 to 17.6 ng/mg, and ecgonine methyl ester traces below the calibration curve of approximately 12.9 ng/mg. In 95% of the cases cocaine exceeded BZE and EME in concentration.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0146-4760
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
16
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
291-6
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1294835-Adolescent,
pubmed-meshheading:1294835-Adult,
pubmed-meshheading:1294835-Aged,
pubmed-meshheading:1294835-Coca,
pubmed-meshheading:1294835-Cocaine,
pubmed-meshheading:1294835-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:1294835-Hair,
pubmed-meshheading:1294835-Humans,
pubmed-meshheading:1294835-Mastication,
pubmed-meshheading:1294835-Middle Aged,
pubmed-meshheading:1294835-Plants, Medicinal,
pubmed-meshheading:1294835-Reproducibility of Results,
pubmed-meshheading:1294835-Sensitivity and Specificity,
pubmed-meshheading:1294835-Substance Abuse Detection
|
| pubmed:articleTitle |
Identification and quantitation of cocaine and its metabolites, benzoylecgonine and ecgonine methyl ester, in hair of Bolivian coca chewers by gas chromatography/mass spectrometry.
|
| pubmed:affiliation |
Institute of Legal Medicine, State University of Saarland, Homburg, Germany.
|
| pubmed:publicationType |
Journal Article
|