Source:http://linkedlifedata.com/resource/pubmed/id/12946649
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2003-8-29
|
| pubmed:abstractText |
The mRNA expression profile in control and clofibric acid (CLO)-treated mouse, rat, and human hepatocytes was analyzed using species-specific oligonucleotide DNA microarrays (Affymetrix). A statistical empirical Bayes procedure was applied in order to select the significantly differentially expressed genes. Treatment with the peroxisome proliferator CLO induced up-regulation of genes involved in peroxisome proliferation and in cell proliferation as well as down-regulation of genes involved in apoptosis in hepatocytes of rodent but not of human origin. CLO treatment induced up-regulation of microsomal cytochrome P450 4a genes in rodent hepatocytes and in two of six human hepatocyte cultures. In addition, genes encoding phenobarbital-inducible cytochrome P450s were also up-regulated by CLO in rodent and human hepatocyte cultures. Up-regulation of phenobarbital-inducible UDP-glucuronosyl-transferase genes by CLO was observed in both rat and human but not in mouse hepatocytes. CLO treatment induced up-regulation of L-fatty acid binding protein (L-FABP) gene in hepatocytes of both rodent and human origin. However, while genes of the cytosolic, microsomal, and mitochondrial pathways involved in fatty acid transport and metabolism were up-regulated by CLO in both rodent and human hepatocyte cultures, genes of the peroxisomal pathway of lipid metabolism were up-regulated in rodents only. An up-regulation of hepatocyte nuclear factor 1alpha (HNF1alpha) by CLO was observed only in human hepatocyte cultures, suggesting that this trans-activating factor may play a key role in the regulation of fatty acid metabolism in human liver as well as in the nonresponsiveness of human liver to CLO-induced regulation of cell proliferation and apoptosis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
|
| pubmed:issn |
0041-008X
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| pubmed:author |
pubmed-author:ChiboutSalah-DineSD,
pubmed-author:GrassPeterP,
pubmed-author:HartmannNicoleN,
pubmed-author:HeydBrunoB,
pubmed-author:LamboleyChristelleC,
pubmed-author:LaurentStephaneS,
pubmed-author:MantionGeorgesG,
pubmed-author:RichertLysianeL,
pubmed-author:StaedtlerFrankF,
pubmed-author:Viollon-AbadieCatherineC
|
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
191
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
130-46
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:12946649-Aged,
pubmed-meshheading:12946649-Animals,
pubmed-meshheading:12946649-Apoptosis,
pubmed-meshheading:12946649-Biological Transport,
pubmed-meshheading:12946649-Cell Membrane,
pubmed-meshheading:12946649-Clofibric Acid,
pubmed-meshheading:12946649-Down-Regulation,
pubmed-meshheading:12946649-Fatty Acids,
pubmed-meshheading:12946649-Female,
pubmed-meshheading:12946649-Gene Expression Regulation,
pubmed-meshheading:12946649-Hepatocytes,
pubmed-meshheading:12946649-Humans,
pubmed-meshheading:12946649-Hypolipidemic Agents,
pubmed-meshheading:12946649-Male,
pubmed-meshheading:12946649-Mice,
pubmed-meshheading:12946649-Middle Aged,
pubmed-meshheading:12946649-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:12946649-RNA, Messenger,
pubmed-meshheading:12946649-Rats,
pubmed-meshheading:12946649-Species Specificity,
pubmed-meshheading:12946649-Up-Regulation
|
| pubmed:year |
2003
|
| pubmed:articleTitle |
Effects of clofibric acid on mRNA expression profiles in primary cultures of rat, mouse and human hepatocytes.
|
| pubmed:affiliation |
Laboratoire de Biologie Cellulaire, UFR SMP, 4, place Saint-Jacques, 25030 Besançon, France. lysiane.richert@univ-fcomte.fr
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| pubmed:publicationType |
Journal Article,
In Vitro
|