Linked Life Data

12946534

Source:http://linkedlifedata.com/resource/pubmed/id/12946534

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-8-29
pubmed:abstractText
Methods using TLC densitometry with fluorescence detection are described for the assay and purity control of tetracycline (TC), chlortetracycline (CTC), and oxytetracycline (OTC) in animal feeds and premixes. With a silica gel layer previously sprayed with 10% (m/v) sodium EDTA solution adjusted to pH 8.0 or 9.0, all the major impurities were separated from the main components and from each other. The mobile phase consisted of dichloromethane, methanol, and water. After development, the plate was dipped in a 30% (v/v) solution of liquid paraffin in hexane. Quantitation was realized by fluorescence densitometry at 400 nm. The limit of quantitation (LOQ) for tetracycline impurities is 0.8 microg/g, corresponding to 0.2% of the label claimed tetracycline (400 microg/g). The LOQ for impurities of tetracycline and chlortetracycline in premixes is 0.2% of the label-claimed TC (40 mg/g) and CTC (200 or 400 mg/g). The LOQ for impurities of oxytetracycline in a premix is 0.1% of the label claimed OTC (100 mg/g).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0731-7085
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
85-93
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Assay and purity control of tetracycline, chlortetracycline and oxytetracycline in animal feeds and premixes by TLC densitometry with fluorescence detection.
pubmed:affiliation
Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Van Evenstraat 4, B-3000 Leuven, Belgium.
pubmed:publicationType
Journal Article