Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-8-27
pubmed:abstractText
In this study, we established a unique cell line, HPB-AML-I (AML-I), from peripheral blood mononuclear cells collected from a patient with acute myeloid leukemia (AML: M1). Morphological and phenotypical analyses of the established AML-I cells demonstrated that they belong to a preadipocyte cell line as indicated by their storage of lipid droplets and expression of surface antigens similar to those found on bone marrow stromal cells (MSC). Through cell culture under adipogenic conditions, effective differentiation of AML-I cells into adipocytes was induced by an adipogenesis inducing cocktail (INC) made up of a mixture of methylisobutylxanthine, hydrocortisone, and indomethacin. By contrast, activation of peroxisome proliferator-activated receptor (PPARgamma), which plays a key role in lipids metabolism and is highly expressed in AML-I cells, decreased the number of lipid droplets in AML-I cells. Here we report the establishment of a unique human derived-preadipocyte cell line, AML-I, and its bi-directional adipogenic response to different type stimulation, i.e., one is a refractory response to troglitazone, a well-known adipogenic stimulator, and a positive response to INC, an adipogenesis induction cocktail. These results suggest that, based on the adipogenic response, there might be some distinct lineages in human adipocytes and that the unique differentiation of AML-I cells should be useful for analyzing both the differentiation and regulation of human preadipocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents, http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents..., http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD, http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents, http://linkedlifedata.com/resource/pubmed/chemical/Chromans, http://linkedlifedata.com/resource/pubmed/chemical/Hydrocortisone, http://linkedlifedata.com/resource/pubmed/chemical/Indomethacin, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear, http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles, http://linkedlifedata.com/resource/pubmed/chemical/Thiazolidinediones, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/troglitazone
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9541
pubmed:author
pubmed:copyrightInfo
Copyright 2003 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
197
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
42-52
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:12942539-Adipocytes, pubmed-meshheading:12942539-Anti-Inflammatory Agents, pubmed-meshheading:12942539-Anti-Inflammatory Agents, Non-Steroidal, pubmed-meshheading:12942539-Antigens, CD, pubmed-meshheading:12942539-Antineoplastic Agents, pubmed-meshheading:12942539-Cell Differentiation, pubmed-meshheading:12942539-Cell Division, pubmed-meshheading:12942539-Cell Line, pubmed-meshheading:12942539-Chromans, pubmed-meshheading:12942539-Flow Cytometry, pubmed-meshheading:12942539-Fluorescent Antibody Technique, pubmed-meshheading:12942539-Humans, pubmed-meshheading:12942539-Hydrocortisone, pubmed-meshheading:12942539-Immunohistochemistry, pubmed-meshheading:12942539-Inclusion Bodies, pubmed-meshheading:12942539-Indomethacin, pubmed-meshheading:12942539-Leukemia, Myeloid, Acute, pubmed-meshheading:12942539-Leukocytes, Mononuclear, pubmed-meshheading:12942539-Microscopy, Electron, pubmed-meshheading:12942539-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:12942539-Thiazoles, pubmed-meshheading:12942539-Thiazolidinediones, pubmed-meshheading:12942539-Transcription Factors
pubmed:year
2003
pubmed:articleTitle
Establishment of a human preadipose cell line, HPB-AML-I: refractory to PPARgamma-mediated adipogenic stimulation.
pubmed:affiliation
Department of Pathology, 1st Unit, Shimane Medical University, Izumo, Japan. ikuko@shimane-med.ac.jp
pubmed:publicationType
Journal Article