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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2003-10-9
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pubmed:abstractText |
Conjugative transfer of F-like plasmids is a tightly regulated process. The TraJ protein is the main positive activator of the tra operon which encodes products required for conjugative transfer of F-like plasmids. Nucleotide sequence analysis revealed potential Lrp and H-NS binding sites in the traJ regulatory region. Expression of a traJ-lacZ fusion in hns and lrp mutant strains showed that both are positive modulators of traJ expression. Competitive RT-PCR demonstrated that H-NS and Lrp exert their effect at the transcriptional level. Electrophoretic mobility-shift assays showed that H-NS and Lrp proteins bind to the traJ promoter. Conjugative transfer of pRK100 was decreased in hns but not in lrp mutant strains. Together, the results indicate H-NS and Lrp function as activators of traJ transcription.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/H-NS protein, bacteria,
http://linkedlifedata.com/resource/pubmed/chemical/Leucine-Responsive Regulatory...,
http://linkedlifedata.com/resource/pubmed/chemical/Lrp protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1617-4615
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
270
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
94-102
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12942368-Bacterial Proteins,
pubmed-meshheading:12942368-Base Sequence,
pubmed-meshheading:12942368-Crosses, Genetic,
pubmed-meshheading:12942368-DNA-Binding Proteins,
pubmed-meshheading:12942368-Escherichia coli,
pubmed-meshheading:12942368-Escherichia coli Proteins,
pubmed-meshheading:12942368-Gene Expression Regulation, Bacterial,
pubmed-meshheading:12942368-Kinetics,
pubmed-meshheading:12942368-Leucine-Responsive Regulatory Protein,
pubmed-meshheading:12942368-Molecular Sequence Data,
pubmed-meshheading:12942368-Plasmids,
pubmed-meshheading:12942368-Proteins,
pubmed-meshheading:12942368-RNA, Bacterial,
pubmed-meshheading:12942368-Recombinant Fusion Proteins,
pubmed-meshheading:12942368-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12942368-Transcription Factors,
pubmed-meshheading:12942368-beta-Galactosidase
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pubmed:year |
2003
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pubmed:articleTitle |
H-NS and Lrp serve as positive modulators of traJ expression from the Escherichia coli plasmid pRK100.
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pubmed:affiliation |
Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, 1000 Ljubljana, Slovenia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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