Linked Life Data

12941609

Source:http://linkedlifedata.com/resource/pubmed/id/12941609

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-8-27
pubmed:abstractText
Myb-binding protein 1a (Mybbp1a) is a novel nuclear protein localized predominantly, but not exclusively, in nucleoli. Although initially isolated as a c-Myb interacting protein, Mybbp1a is expressed ubiquitously, associates with a number of different transcription factors, and may play a role in both RNA polymerase I- and II-mediated transcriptional regulation. However, its precise function remains unclear. In this study we show that Mybbp1a is a nucleocytoplasmic shuttling protein and investigate the mechanisms responsible for both nuclear import and export. The carboxyl terminus of Mybbp1a, which contains seven short basic amino acid repeat sequences, is responsible for both nuclear and nucleolar localization, and this activity can be transferred to a heterologous protein. Deletion mapping demonstrated that these repeat sequences appear to act incrementally, with successive deletions resulting in a corresponding increase in the proportion of protein localized in the cytoplasm. Glutathione S-transferase pulldown experiments showed that the nuclear receptor importin-alpha/beta mediates Mybbp1a nuclear import. Interspecies heterokaryons were used to demonstrate that Mybbp1a was capable of shuttling between the nucleus and the cytoplasm. Deletion analysis and in vivo export studies using a heterologous assay system identified several nuclear export sequences which facilitate Mybbp1a nuclear export of Mybbp1a by CRM1-dependent and -independent pathways.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0014-4827
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
289
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
108-23
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12941609-3T3 Cells, pubmed-meshheading:12941609-Active Transport, Cell Nucleus, pubmed-meshheading:12941609-Amino Acid Sequence, pubmed-meshheading:12941609-Animals, pubmed-meshheading:12941609-Base Sequence, pubmed-meshheading:12941609-Carrier Proteins, pubmed-meshheading:12941609-Cell Nucleolus, pubmed-meshheading:12941609-Cell Nucleus, pubmed-meshheading:12941609-Cytoplasm, pubmed-meshheading:12941609-Fibroblasts, pubmed-meshheading:12941609-Humans, pubmed-meshheading:12941609-Karyopherins, pubmed-meshheading:12941609-Mice, pubmed-meshheading:12941609-Molecular Sequence Data, pubmed-meshheading:12941609-Nuclear Proteins, pubmed-meshheading:12941609-Nucleocytoplasmic Transport Proteins, pubmed-meshheading:12941609-Protein Structure, Tertiary, pubmed-meshheading:12941609-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:12941609-alpha Karyopherins
pubmed:year
2003
pubmed:articleTitle
Myb-binding protein 1a is a nucleocytoplasmic shuttling protein that utilizes CRM1-dependent and independent nuclear export pathways.
pubmed:affiliation
Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide, South Australia 5000, Australia. rebecca.keough@adelaide.edu.au
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't