Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0001801,
umls-concept:C0014441,
umls-concept:C0020985,
umls-concept:C0022885,
umls-concept:C0023117,
umls-concept:C0026941,
umls-concept:C0036745,
umls-concept:C0220825,
umls-concept:C0220911,
umls-concept:C0282646,
umls-concept:C0301911,
umls-concept:C0392366,
umls-concept:C0680536,
umls-concept:C1707455
|
| pubmed:issue |
8
|
| pubmed:dateCreated |
1993-4-7
|
| pubmed:abstractText |
A panel of 68 serum specimens from 41 subjects exhibiting various immunological patterns to Mycoplasma pneumoniae as determined by detection of a 180 kDa protein in immunoblotting was used to compare five commercially available tests based on different methods: complement fixation test (CFT), microparticle agglutination (MAG), indirect immunofluorescence assay (IFA), enzyme-linked immunosorbent assay (Elisa), and latex agglutination (LA). The tests were performed according to the manufacturers' instructions. For the determination of immunity to M pneumoniae, the five tests were in good accordance with immunoblotting: sensitivity was 100% for all the five assays, specificity ranged from 95.6% (MAG) to 82.6% (Elisa) and overall agreement ranged from 98.2% (MAG) to 92.8% (Elisa). The comparisons of antibody rates obtained by the four quantitative tests (CFT, MAG, IFA, Elisa) showed correlation coefficients ranging from 0.87 (CFT-IFA) to 0.67 (CFT-Elisa). Six significant antibody rises demonstrated by immunoblotting patterns were detected by all the tests but Elisa in one case. As a whole, the commercial assays gave satisfactory results for routine determination of immune status to M pneumoniae: CFT was the cheapest test and MAG and LA were the easiest to perform.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0003-3898
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
50
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
593-7
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1294015-Adolescent,
pubmed-meshheading:1294015-Adult,
pubmed-meshheading:1294015-Agglutination Tests,
pubmed-meshheading:1294015-Antibodies, Bacterial,
pubmed-meshheading:1294015-Child,
pubmed-meshheading:1294015-Child, Preschool,
pubmed-meshheading:1294015-Complement Fixation Tests,
pubmed-meshheading:1294015-Diagnostic Tests, Routine,
pubmed-meshheading:1294015-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1294015-Female,
pubmed-meshheading:1294015-Fluorescent Antibody Technique,
pubmed-meshheading:1294015-Humans,
pubmed-meshheading:1294015-Immunoblotting,
pubmed-meshheading:1294015-Immunosorbent Techniques,
pubmed-meshheading:1294015-Infant,
pubmed-meshheading:1294015-Latex Fixation Tests,
pubmed-meshheading:1294015-Male,
pubmed-meshheading:1294015-Middle Aged,
pubmed-meshheading:1294015-Mycoplasma pneumoniae
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Evaluation of five commercial tests: complement fixation, microparticle agglutination, indirect immunofluorescence, enzyme-linked immunosorbent assay and latex agglutination, in comparison to immunoblotting for Mycoplasma pneumoniae serology.
|
| pubmed:affiliation |
Laboratoire de Bactériologie-Virologie, Faculté de Médecine Jacques-Lisfranc, Université de Saint-Etienne, France.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|