Linked Life Data

12938327

Source:http://linkedlifedata.com/resource/pubmed/id/12938327

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-8-26
pubmed:abstractText
In this paper, we study the enzyme catalysis reaction kinetics that 2,3-diaminophenazine compound was synthesized with the horseradish peroxidase catalyzing reaction of H2O2 oxidizing o-phenylemediamine. First, the product of oxidation of o-phenylenediamine (OPD) by H2O2 catalyzed and by horseradish peroxidase (HRP) was synthesized. The finest reaction conditions (acid degree, temperature, OPD, H2O2, HRP various molar ratio) were selected. The reaction conditions and the product were determined by UV-absorption spectrum, IR and element analysis. The results showed that the product is 2,3-diaminophenazine. Secondly, the reaction order of OPD-H2O2-HRP system and the rate constant were studied by using ultraviolet spectrophotometry based on Michaelis-Menten equation. The finest conditions lambda max = 446.3 nm, pH = 4.85, t = 22 degrees C were selected, a series experiments were done. Experimental results showed that this is an one reactant reaction determined by the experiment and theory, and derived K(m) = 6.02 x 10(-3) mol.L-1 and upsilon m = 7.2 x 10(-2) delta A.min-1.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1000-0593
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
436-40
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
[The study on reaction kinetics based on a new system of the horseradish peroxidase catalyting the oxidation of o-phenylenediamine by H2O2].
pubmed:affiliation
Department of Chemistry, Liaoning Normal University, Dalian 116029, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't