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pubmed-article:1293763pubmed:abstractTextMembrane properties of the fully-grown oocytes from toad, Bufo bufo gargarizans, were studied by using voltage-clamp technique. It was found that a sustained outward current was elicited by membrane depolarization to -30 mV or more positive value. The increase of the current was nearly proportional to the degree of depolarization. The peak value of the current ranged 2-5 microA at a membrane potential of 20 mV in oocytes from different toads. The current was inhibited by antagonists of potassium channel, TEA and 4-AP. The concentration of TEA capable of inhibiting half of the current was 2.6 mmol/L. Chloride channel antagonist 9-AC (2.5 mmol/L) had no effect on the current. Triple the extracellular calcium concentration did not show any effect either. The reversal potential of the current varied with an increase of 47.3 mV per decade change of the extracellular potassium concentration. Changing extracellular concentration of sodium or chloride did not shift the reversal potential. It was concluded that the outward current was a voltage-activated potassium current. The voltage-dependent potassium current decreased after treatment of the oocytes with progesterone to a state of maturation. A large decrease of the current (to about 1/20 of the control) occurred to the oocytes obtained from hibernating toads while a less striking decrease of the current (to about 1/3 of the control) was observed in the oocytes from toads all year round reared at 25-30 degrees C.lld:pubmed
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pubmed-article:1293763pubmed:authorpubmed-author:BaoY DYDlld:pubmed
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pubmed-article:1293763pubmed:pagination461-9lld:pubmed
pubmed-article:1293763pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:1293763pubmed:articleTitle[A voltage-dependent potassium channel of outward rectifier type in plasma membrane of oocyte from toad, Bufo bufo gargarizans].lld:pubmed
pubmed-article:1293763pubmed:affiliationShanghai Institute of Physiology.lld:pubmed
pubmed-article:1293763pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1293763pubmed:publicationTypeEnglish Abstractlld:pubmed