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rdf:type |
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lifeskim:mentions |
umls-concept:C0003232,
umls-concept:C0004611,
umls-concept:C0018150,
umls-concept:C0024432,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0127400,
umls-concept:C0205263,
umls-concept:C0333348,
umls-concept:C0443199,
umls-concept:C0443348,
umls-concept:C0871261,
umls-concept:C1363844,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1709059,
umls-concept:C2911692
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pubmed:issue |
4
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pubmed:dateCreated |
2003-8-25
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pubmed:abstractText |
We have investigated effects of beta-lactam antibiotics on TNF-alpha, and iNOS production from mouse peritoneal macrophages following co-culture with Escherichia coli or Staphylococcus aureus bacteria. Ceftazidime and aztreonam enhanced TNF-alpha secretion from macrophages stimulated with E. coli; however, imipenem does not alter either the kinetics or magnitude of TNF-alpha in E. coli-treated macrophages. Similar treatments with S. aureus co-cultured with macrophages markedly altered profiles of TNF-alpha response characterized by apparent early TNF-alpha peak relative to untreated S. aureus. All antibiotics increased E. coli-induced iNOS expression as assessed by both mRNA and protein. These same antibiotics significantly reduced S. aureus-induced iNOS levels of RNA. Both ceftazidime and aztreonam enhanced LPS release from E. coli in comparison to low-level LPS release from imipenem-treated bacteria, consistent with observed differences in TNF-alpha release. Incubation of all three antibiotics with S. aureus similarly increased levels of the cell wall constituent protein A detected in supernatants at early time points indicating microbial lysis. In parallel, S. aureus culture supernatants from 2-h incubation with antibiotics enhanced TNF-alpha release. These results indicate that different cellular mechanisms contribute to antibiotic-mediated regulation of TNF-alpha and iNOS secretion in mouse macrophages in response to E. coli versus S. aureus.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Bacterial Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Aztreonam,
http://linkedlifedata.com/resource/pubmed/chemical/Ceftazidime,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Imipenem,
http://linkedlifedata.com/resource/pubmed/chemical/Inflammation Mediators,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Staphylococcal Protein A,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:issn |
0968-0519
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
225-36
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12935353-Animals,
pubmed-meshheading:12935353-Anti-Bacterial Agents,
pubmed-meshheading:12935353-Aztreonam,
pubmed-meshheading:12935353-Ceftazidime,
pubmed-meshheading:12935353-Cells, Cultured,
pubmed-meshheading:12935353-Coculture Techniques,
pubmed-meshheading:12935353-Culture Media, Conditioned,
pubmed-meshheading:12935353-Escherichia coli,
pubmed-meshheading:12935353-Female,
pubmed-meshheading:12935353-Imipenem,
pubmed-meshheading:12935353-Inflammation Mediators,
pubmed-meshheading:12935353-Macrophages, Peritoneal,
pubmed-meshheading:12935353-Mice,
pubmed-meshheading:12935353-Mice, Inbred C3H,
pubmed-meshheading:12935353-Nitric Oxide Synthase,
pubmed-meshheading:12935353-Nitric Oxide Synthase Type II,
pubmed-meshheading:12935353-RNA, Messenger,
pubmed-meshheading:12935353-Staphylococcal Protein A,
pubmed-meshheading:12935353-Staphylococcus aureus,
pubmed-meshheading:12935353-Tumor Necrosis Factor-alpha
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pubmed:year |
2003
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pubmed:articleTitle |
Differential modulation of the induction of inflammatory mediators by antibiotics in mouse macrophages in response to viable Gram-positive and Gram-negative bacteria.
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pubmed:affiliation |
Department of Basic Medical Science, University of Missouri-Kansas City School of Medicine, Kansas City, MO 64108, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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