Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2003-9-3
pubmed:abstractText
By considering temperature effects on the mechanical unfolding rates of proteins and RNA, whose energy landscape is rugged, the question posed in the title is answered in the affirmative. Adopting a theory by Zwanzig [Zwanzig, R. (1988) Proc. Natl. Acad. Sci. USA 85, 2029-2030], we show that, because of roughness characterized by an energy scale epsilon, the unfolding rate at constant force is retarded. Similarly, in nonequilibrium experiments done at constant loading rates, the most probable unfolding force increases because of energy landscape roughness. The effects are dramatic at low temperatures. Our analysis suggests that, by using temperature as a variable in mechanical unfolding experiments of proteins and RNA, the ruggedness energy scale epsilon, can be directly measured.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12934020-10639131, http://linkedlifedata.com/resource/pubmed/commentcorrection/12934020-10860987, http://linkedlifedata.com/resource/pubmed/commentcorrection/12934020-10860990, http://linkedlifedata.com/resource/pubmed/commentcorrection/12934020-11274384, http://linkedlifedata.com/resource/pubmed/commentcorrection/12934020-11297932, http://linkedlifedata.com/resource/pubmed/commentcorrection/12934020-11326101, http://linkedlifedata.com/resource/pubmed/commentcorrection/12934020-12052949, http://linkedlifedata.com/resource/pubmed/commentcorrection/12934020-12649482, http://linkedlifedata.com/resource/pubmed/commentcorrection/12934020-3353365, http://linkedlifedata.com/resource/pubmed/commentcorrection/12934020-8989315, http://linkedlifedata.com/resource/pubmed/commentcorrection/12934020-9083660, http://linkedlifedata.com/resource/pubmed/commentcorrection/12934020-9148805, http://linkedlifedata.com/resource/pubmed/commentcorrection/12934020-9153398
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
100
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10249-53
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Can energy landscape roughness of proteins and RNA be measured by using mechanical unfolding experiments?
pubmed:affiliation
Chemical Physics Program, Institute for Physical Science and Technology, University of Maryland, College Park, MD 20742, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.