Linked Life Data

12933821

Source:http://linkedlifedata.com/resource/pubmed/id/12933821

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
45
pubmed:dateCreated
2003-11-3
pubmed:abstractText
The protein product of the CHI3L1 gene, human cartilage 39-kDa glycoprotein (HC-gp39), is a tissue-restricted, chitin-binding lectin and member of glycosyl hydrolase family 18. In contrast to many other monocyte/macrophage markers, its expression is absent in monocytes and strongly induced during late stages of human macrophage differentiation. To gain insights into the molecular mechanisms underlying its cell type-restricted and maturation-associated expression in macrophages, we initiated a detailed study of the proximal HC-gp39 promoter. Deletion analysis of reporter constructs in macrophage-like THP-1 cells localized a region directing high levels of macrophage-specific reporter gene expression to approximately 300 bp adjacent to the major transcriptional start site. The promoter sequence contained consensus binding sites for several known factors, and specific binding of nuclear PU.1, Sp1, Sp3, USF, AML-1, and C/EBP proteins was detectable in gel shift assays. In vivo footprinting assays with dimethyl sulfate demonstrate that the protection of corresponding sequences was enhanced in macrophages compared with monocytes. Mutational analysis of transcription factor binding sites indicated a predominant role for a single Sp1 binding site in regulating HC-gp39 promoter activity. In addition, gel shift assays using nuclear extracts of monocytes and macrophages demonstrated that the binding of nuclear Sp1, but not Sp3, markedly increases during macrophage differentiation. Our results further highlight the important role of Sp1 in macrophage gene regulation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
278
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
44058-67
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:12933821-Adipokines, pubmed-meshheading:12933821-Animals, pubmed-meshheading:12933821-Base Sequence, pubmed-meshheading:12933821-Binding Sites, pubmed-meshheading:12933821-Cell Differentiation, pubmed-meshheading:12933821-Cell Line, pubmed-meshheading:12933821-Cell Nucleus, pubmed-meshheading:12933821-Consensus Sequence, pubmed-meshheading:12933821-DNA, pubmed-meshheading:12933821-DNA Footprinting, pubmed-meshheading:12933821-Drosophila, pubmed-meshheading:12933821-Electrophoretic Mobility Shift Assay, pubmed-meshheading:12933821-Gene Deletion, pubmed-meshheading:12933821-Gene Expression, pubmed-meshheading:12933821-Gene Expression Regulation, pubmed-meshheading:12933821-Glycoproteins, pubmed-meshheading:12933821-Humans, pubmed-meshheading:12933821-Lectins, pubmed-meshheading:12933821-Macrophages, pubmed-meshheading:12933821-Molecular Sequence Data, pubmed-meshheading:12933821-Mutagenesis, pubmed-meshheading:12933821-Promoter Regions, Genetic, pubmed-meshheading:12933821-Sp1 Transcription Factor, pubmed-meshheading:12933821-Tetradecanoylphorbol Acetate, pubmed-meshheading:12933821-Transcription, Genetic, pubmed-meshheading:12933821-Transcription Factors, pubmed-meshheading:12933821-Transfection
pubmed:year
2003
pubmed:articleTitle
Transcriptional regulation of CHI3L1, a marker gene for late stages of macrophage differentiation.
pubmed:affiliation
Department of Hematology and Oncology, University of Regensburg, 93042 Regensburg, Germany. michael.rehli@klinik.uni-regensburg.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't