Source:http://linkedlifedata.com/resource/pubmed/id/12932653
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2003-8-22
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| pubmed:abstractText |
Acid-terminated polyglycolide microparticles (PG-MP) were prepared as a versatile substrate that could be surface-modified for either immobilization of anti-cd3 and anti-cd28 mAb to activate T cells or sustained release of granulocyte-macrophage colony stimulating factor (GM-CSF) for dendritic cell (DC) recruitment and maturation. PG-MP were prepared with a volume-weighted mean diameter of 56 or 57 microm. Accessible carboxylic acid group concentration was determined by potentiometric titration to be 0.3 mmole/g and corresponded to a zeta potential of -21.87 mV. PG-MP immobilized with either anti-human CD3/CD28 or anti-mouse cd3/cd28 induced significant proliferation of T cells. Intracellular flow cytometry in activated mouse T cells was significant for IFN-gamma, but not IL-4. Microparticles surface-modified for GM-CSF release were prepared from either PG-MP or PG pre-treated with poly-L-lysine (PG-Lys) to manipulate surface charge. GM-CSF released from PG-Lys-MP was observed for up to 26 days. The biologic activity of released GM-CSF was confirmed by using a h-GM-CSF-dependent cell line. The efficacy of the alpha-cd3/cd28-MP and GMCSF-MP was studied in a syngeneic mouse tumor prevention and regression model. Co-injection of Meth A fibrosarcoma cells with alpha-cd3/cd28-MP and GMCSF-MP completely prevented tumor implantation (0/24). The regression model showed complete tumor regression in four of seven animals and stable disease in three of seven. In the latter study, a dramatic level of DC infiltration was observed compared to controls.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Polyglycolic Acid
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0168-3659
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
28
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| pubmed:volume |
91
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
209-24
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:12932653-Animals,
pubmed-meshheading:12932653-Antigens, CD28,
pubmed-meshheading:12932653-Antigens, CD3,
pubmed-meshheading:12932653-Cytokines,
pubmed-meshheading:12932653-Dendritic Cells,
pubmed-meshheading:12932653-Flow Cytometry,
pubmed-meshheading:12932653-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:12932653-Humans,
pubmed-meshheading:12932653-Immunotherapy,
pubmed-meshheading:12932653-Lymphocyte Activation,
pubmed-meshheading:12932653-Mice,
pubmed-meshheading:12932653-Mice, Inbred BALB C,
pubmed-meshheading:12932653-Microscopy, Electron,
pubmed-meshheading:12932653-Microspheres,
pubmed-meshheading:12932653-Neoplasm Transplantation,
pubmed-meshheading:12932653-Neoplasms,
pubmed-meshheading:12932653-Polyglycolic Acid,
pubmed-meshheading:12932653-T-Lymphocytes
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| pubmed:year |
2003
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| pubmed:articleTitle |
Development of novel substrates for tumor immunotherapy.
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| pubmed:affiliation |
Division of Gynecologic Oncology, University of Pennsylvania Medical Center, 3400 Spruce Street, First Floor Dulles, Suite 1000 Courtyard, Philadelphia, PA 19104, USA. wsalaby@mail.obgyn.upenn.edu
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| pubmed:publicationType |
Journal Article
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