| pubmed-article:12930846 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12930846 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:12930846 | lifeskim:mentions | umls-concept:C0682323 | lld:lifeskim |
| pubmed-article:12930846 | lifeskim:mentions | umls-concept:C0072108 | lld:lifeskim |
| pubmed-article:12930846 | lifeskim:mentions | umls-concept:C0032403 | lld:lifeskim |
| pubmed-article:12930846 | lifeskim:mentions | umls-concept:C0249197 | lld:lifeskim |
| pubmed-article:12930846 | lifeskim:mentions | umls-concept:C0678640 | lld:lifeskim |
| pubmed-article:12930846 | pubmed:issue | 41 | lld:pubmed |
| pubmed-article:12930846 | pubmed:dateCreated | 2003-10-6 | lld:pubmed |
| pubmed-article:12930846 | pubmed:abstractText | We addressed the analysis of the physical and functional association of proliferating cell nuclear antigen (PCNA), a protein involved in many DNA transactions, with poly(ADP-ribose) polymerase (PARP-1), an enzyme that plays a crucial role in DNA repair and interacts with many DNA replication/repair factors. We demonstrated that PARP-1 and PCNA co-immunoprecipitated both from the soluble and the DNA-bound fraction isolated from S-phase-synchronized HeLa cells. Immunoprecipitation experiments with purified proteins further confirmed a physical association between PARP-1 and PCNA. To investigate the effect of this association on PARP-1 activity, an assay based on the incorporation of radioactive NAD was performed. Conversely, the effect of PARP-1 on PCNA-dependent DNA synthesis was assessed by a DNA polymerase delta assay. A marked inhibition of both reactions was found. Unexpectedly, PARP-1 activity also decreased in the presence of p21waf1/cip1. By pull-down experiments, we provided the first evidence for an association between PARP-1 and p21, which involves the C-terminal part of p21 protein. This association was further demonstrated to occur also in vivo in MNNG (N-methyl-N'-nitro-N-nitrosoguanidine)-treated human fibroblasts. These observations suggest that PARP-1 and p21 could cooperate in regulating the functions of PCNA during DNA replication/repair. | lld:pubmed |
| pubmed-article:12930846 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12930846 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12930846 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12930846 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12930846 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12930846 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12930846 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12930846 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12930846 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12930846 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12930846 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12930846 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12930846 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:12930846 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:12930846 | pubmed:author | pubmed-author:MagaGiovanniG | lld:pubmed |
| pubmed-article:12930846 | pubmed:author | pubmed-author:SpadariSilvio... | lld:pubmed |
| pubmed-article:12930846 | pubmed:author | pubmed-author:FrouinIsabell... | lld:pubmed |
| pubmed-article:12930846 | pubmed:author | pubmed-author:DenegriMarcoM | lld:pubmed |
| pubmed-article:12930846 | pubmed:author | pubmed-author:ScovassiA... | lld:pubmed |
| pubmed-article:12930846 | pubmed:author | pubmed-author:RivaFedericaF | lld:pubmed |
| pubmed-article:12930846 | pubmed:author | pubmed-author:SavioMonicaM | lld:pubmed |
| pubmed-article:12930846 | pubmed:author | pubmed-author:ProsperiEnnio... | lld:pubmed |
| pubmed-article:12930846 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12930846 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:12930846 | pubmed:volume | 278 | lld:pubmed |
| pubmed-article:12930846 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12930846 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12930846 | pubmed:pagination | 39265-8 | lld:pubmed |
| pubmed-article:12930846 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12930846 | pubmed:meshHeading | pubmed-meshheading:12930846... | lld:pubmed |
| pubmed-article:12930846 | pubmed:meshHeading | pubmed-meshheading:12930846... | lld:pubmed |
| pubmed-article:12930846 | pubmed:meshHeading | pubmed-meshheading:12930846... | lld:pubmed |
| pubmed-article:12930846 | pubmed:meshHeading | pubmed-meshheading:12930846... | lld:pubmed |
| pubmed-article:12930846 | pubmed:meshHeading | pubmed-meshheading:12930846... | lld:pubmed |
| pubmed-article:12930846 | pubmed:meshHeading | pubmed-meshheading:12930846... | lld:pubmed |
| pubmed-article:12930846 | pubmed:meshHeading | pubmed-meshheading:12930846... | lld:pubmed |
| pubmed-article:12930846 | pubmed:meshHeading | pubmed-meshheading:12930846... | lld:pubmed |
| pubmed-article:12930846 | pubmed:meshHeading | pubmed-meshheading:12930846... | lld:pubmed |
| pubmed-article:12930846 | pubmed:meshHeading | pubmed-meshheading:12930846... | lld:pubmed |
| pubmed-article:12930846 | pubmed:meshHeading | pubmed-meshheading:12930846... | lld:pubmed |
| pubmed-article:12930846 | pubmed:meshHeading | pubmed-meshheading:12930846... | lld:pubmed |
| pubmed-article:12930846 | pubmed:meshHeading | pubmed-meshheading:12930846... | lld:pubmed |
| pubmed-article:12930846 | pubmed:meshHeading | pubmed-meshheading:12930846... | lld:pubmed |
| pubmed-article:12930846 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12930846 | pubmed:articleTitle | Human proliferating cell nuclear antigen, poly(ADP-ribose) polymerase-1, and p21waf1/cip1. A dynamic exchange of partners. | lld:pubmed |
| pubmed-article:12930846 | pubmed:affiliation | Istituto di Genetica Molecolare, Consiglio Nazionale delle Ricerche, Via Abbiategrasso 207, 27100 Pavia, Italy. | lld:pubmed |
| pubmed-article:12930846 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12930846 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:142 | entrezgene:pubmed | pubmed-article:12930846 | lld:entrezgene |
| entrez-gene:1026 | entrezgene:pubmed | pubmed-article:12930846 | lld:entrezgene |
| entrez-gene:5111 | entrezgene:pubmed | pubmed-article:12930846 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:12930846 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:12930846 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:12930846 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:12930846 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:12930846 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:12930846 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12930846 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12930846 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12930846 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12930846 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12930846 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12930846 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12930846 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12930846 | lld:pubmed |
