Linked Life Data

12928420

Source:http://linkedlifedata.com/resource/pubmed/id/12928420

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2003-8-20
pubmed:abstractText
We used multiparameter flow cytometry to characterize leukocyte immunophenotypes and cytokines in skin and peripheral blood of patients with erythema migrans (EM). Dermal leukocytes and cytokines were assessed in fluids aspirated from epidermal suction blisters raised over EM lesions and skin of uninfected controls. Compared with corresponding peripheral blood, EM infiltrates were enriched for T cells, monocytes/macrophages, and dendritic cells (DCs), contained lower proportions of neutrophils, and were virtually devoid of B cells. Enhanced expression of CD14 and HLA-DR by lesional neutrophils and macrophages indicated that these innate effector cells were highly activated. Staining for CD45RO and CD27 revealed that lesional T lymphocytes were predominantly Ag-experienced cells; furthermore, a subset of circulating T cells also appeared to be neosensitized. Lesional DC subsets, CD11c(+) (monocytoid) and CD11c(-) (plasmacytoid), expressed activation/maturation surface markers. Patients with multiple EM lesions had greater symptom scores and higher serum levels of IFN-alpha, TNF-alpha, and IL-2 than patients with solitary EM. IL-6 and IFN-gamma were the predominant cytokines in EM lesions; however, greater levels of both mediators were detected in blister fluids from patients with isolated EM. Circulating monocytes displayed significant increases in surface expression of Toll-like receptor (TLR)1 and TLR2, while CD11c(+) DCs showed increased expression of TLR2 and TLR4; lesional macrophages and CD11c(+) and CD11c(-) DCs exhibited increases in expression of all three TLRs. These results demonstrate that Borrelia burgdorferi triggers innate and adaptive responses during early Lyme disease and emphasize the interdependence of these two arms of the immune response in the efforts of the host to contain spirochetal infection.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
171
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2660-70
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12928420-Adult, pubmed-meshheading:12928420-Aged, pubmed-meshheading:12928420-Biological Markers, pubmed-meshheading:12928420-Blister, pubmed-meshheading:12928420-Borrelia burgdorferi, pubmed-meshheading:12928420-Cell Movement, pubmed-meshheading:12928420-Cytokines, pubmed-meshheading:12928420-Dendritic Cells, pubmed-meshheading:12928420-Erythema Chronicum Migrans, pubmed-meshheading:12928420-Female, pubmed-meshheading:12928420-Humans, pubmed-meshheading:12928420-Immunity, Cellular, pubmed-meshheading:12928420-Immunity, Innate, pubmed-meshheading:12928420-Leukocytes, pubmed-meshheading:12928420-Male, pubmed-meshheading:12928420-Membrane Glycoproteins, pubmed-meshheading:12928420-Middle Aged, pubmed-meshheading:12928420-Phagocytes, pubmed-meshheading:12928420-Receptors, Cell Surface, pubmed-meshheading:12928420-Skin, pubmed-meshheading:12928420-T-Lymphocyte Subsets, pubmed-meshheading:12928420-Toll-Like Receptor 1, pubmed-meshheading:12928420-Toll-Like Receptor 2, pubmed-meshheading:12928420-Toll-Like Receptor 4, pubmed-meshheading:12928420-Toll-Like Receptors
pubmed:year
2003
pubmed:articleTitle
Coevolution of markers of innate and adaptive immunity in skin and peripheral blood of patients with erythema migrans.
pubmed:affiliation
Department of Pediatrics, Division of Pediatric Infectious Diseases, Connecticut Children's Medical Center, 282 Washington Street, Hartford, CT 06106, USA. jsalaza@ccmckids.org
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't