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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2003-8-20
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pubmed:abstractText |
Although genetic studies have suggested that heparan sulfate (HS) is involved in bone morphogenetic protein (BMP)-mediated embryonic morphogenesis, it is unclear whether HS is directly involved in BMP-mediated signaling. Here, we investigate the involvement of HS in BMP-7 signaling. We show that HS and heparin chains specifically bind to BMP-7. Digestion of cell-surface HS with heparitinase interferes with BMP-7-mediated Smad phosphorylation in ROS 17/2.8 osteoblastic cells. Inhibiting sulfation of cell-surface HS with chlorate also causes interruption of Smad phosphorylation. Addition of exogenous heparin to ROS 17/2.8 cells prevents BMP-7-mediated Smad phosphorylation rather than enhances the BMP-7 signal, suggesting that HS should be anchored on the plasma membrane for BMP signaling. Moreover, BMP-7 binding to ROS 17/2.8 cells is inhibited by chlorate treatment and exogenous application of heparin. These results demonstrate that BMP-7 specifically binds to cell-surface HS and the BMP-7-HS interaction is required for BMP-7 signaling.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bmp7 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 7,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorine,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosaminoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Heparin,
http://linkedlifedata.com/resource/pubmed/chemical/Heparitin Sulfate,
http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharide-Lyases,
http://linkedlifedata.com/resource/pubmed/chemical/Sepharose,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/heparitinsulfate lyase
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
308
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
858-65
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12927798-Animals,
pubmed-meshheading:12927798-Bone Morphogenetic Protein 7,
pubmed-meshheading:12927798-Bone Morphogenetic Proteins,
pubmed-meshheading:12927798-Cell Membrane,
pubmed-meshheading:12927798-Chlorine,
pubmed-meshheading:12927798-Dose-Response Relationship, Drug,
pubmed-meshheading:12927798-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:12927798-Glycosaminoglycans,
pubmed-meshheading:12927798-Heparin,
pubmed-meshheading:12927798-Heparitin Sulfate,
pubmed-meshheading:12927798-Immunoblotting,
pubmed-meshheading:12927798-Models, Chemical,
pubmed-meshheading:12927798-Osteoblasts,
pubmed-meshheading:12927798-Phosphorylation,
pubmed-meshheading:12927798-Polysaccharide-Lyases,
pubmed-meshheading:12927798-Protein Binding,
pubmed-meshheading:12927798-Rats,
pubmed-meshheading:12927798-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12927798-Sepharose,
pubmed-meshheading:12927798-Signal Transduction,
pubmed-meshheading:12927798-Transforming Growth Factor beta,
pubmed-meshheading:12927798-Tumor Cells, Cultured
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pubmed:year |
2003
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pubmed:articleTitle |
Heparan sulfate is required for bone morphogenetic protein-7 signaling.
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pubmed:affiliation |
The Tokyo Metropolitan Institute of Medical Science, Department of Biochemical Cell Research, Bunkyo-ku, Tokyo 113-8613, Japan. irie@rinshoken.or.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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